食品科学 ›› 2006, Vol. 27 ›› Issue (12): 54-57.

• 基础研究 • 上一篇    下一篇

超声非热处理因素对细菌杀菌效果的影响

周丽珍,李冰,李琳,张喜梅   

  1. 华南理工大学轻工与食品学院轻化工研究所;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Inactivation of Microbes by Non-thermal Ultrasound Sterilization and the Effects of Ultrasound Treatment Factors

ZHOU Li-zhen,LI Bing,LI Lin,ZHANG Xi-mei   

  1. Institute of Ligh Industry and Chemical Engineering, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 本文以大肠杆菌和金黄色葡萄球菌这两种食品中常见的污染菌为研究对象,考察了超声处理条件、介质、温度对超声非热杀菌效果的影响。结果表明:超声处理对大肠杆菌有较强的杀菌作用,金黄色葡萄球菌则对超声非热处理有很强的抵抗力;超声强度增大、作用时间延长、温度升高都有利于杀菌效果的提高;在培养基介质中的杀菌效果强于蒸馏水介质中的情况。结果分析认为,有利于空化作用提高或使细胞强度变弱的条件都有利于提高杀菌效果。冷藏结果表明,杀菌处理后的细菌没有出现复活生长的迹象。

关键词: 超声, 非热杀菌, 杀菌, 平板活菌计数

Abstract: The non-thermal disinfection of Escherichia coli and Staphylococcus aureus by ultrasound treatment were investigated. In this study, the effects of the ultrasound treatment factors, the medium properties and the treatment temperature were evaluated. The results showed that E.coli was susceptible to ultrasound treatment, while S.aureus had great resistance to the treatment. Inactivation of microbes were improved by strengthening power intensity, prolonging irradiation time and increasing temperature. The disinfection of microbes in culture medium was more effective than in distilled water. In a conclusion, everything which could strengthen the cavitation or weaken the cell structure should be effective in disinfection. No cell recovery was found occurring according to the result of viable clone counting after being storaged at 4℃.

Key words: ultrasound, non-thermal sterilization, inactivation, viable plate count