鲫鱼脑乙酰胆碱酯酶(AChE)的活性测定及对有机磷农药的敏感性研究

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 71-74.

鲫鱼脑乙酰胆碱酯酶(AChE)的活性测定及对有机磷农药的敏感性研究

刘晓宇,郝强,吴谋成,杨健,徐盈

华中农业大学食品科技学院；中国水产科学研究院淡水渔业研究中心内陆渔业环境与资源重点开放实验室；中国科学院水生生物研究所；

出版日期:2006-12-15 发布日期:2011-11-23

Study on the Determination of Brain AChE Activity in Crucian Carp and Its Sensitivity to Organophosphorus Pesticides

LIU Xiao-yu,HAO Qiang,WU Mou-cheng,YANG Jian,XU Ying

1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Key Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;3.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 提取不同生长期的鲫鱼脑中乙酰胆碱酯酶(acetylcholinesterase,AChE)并做比较,以Ellman法测定其酶活。并用正交试验方法确定了最佳测定条件。采用体外实验法研究了在最佳条件下鲫鱼脑AChE对三种有机磷农药的敏感性。研究结果表明,以体长12cm左右的鲫鱼体内AChE活性最高,正交试验得出AChE最佳测定条件组合为:酶的剂量为40μl、底物最终浓度为120mmol/L、pH值7.5、温度35℃;时间15min。鲫鱼脑AChE对农药的敏感性为敌敌畏>辛硫磷>乐果。

关键词: 乙酰胆碱酯酶, 正交试验, 有机磷农药, 敏感性

Abstract: Acetylcholinesterase (AChE) was extracted from crucian brain in different growing periods and compared with each other. The activity of AChE was determined by the colorimetric method of Ellman modified by Gorun(1978). Then the optimal conditions were obtained by orthogonal test. Meanwhile, the sensitivity of AChE to three kinds of organophosphorus pesticides was studied under the optimal conditions. The results indicated that the activity of AchE from crucian with the length of 12cm was the best. The optimal conditions by orthogonal test were in the following : the amount of enzyme was 40μl, the concentration of `substrate was 120mmol/L, pH was 7.5, temperature was 35℃ and the reaction time was 15min. The sensitivity of AChE from crucian brain to organophosphorus pesticides was: dicholves>phoxime>dimethoate.

Key words: acetylcholinesterase, orthogonal test, organophosphorus pesticide, sensitivity

刘晓宇,郝强,吴谋成,杨健,徐盈. 鲫鱼脑乙酰胆碱酯酶(AChE)的活性测定及对有机磷农药的敏感性研究[J]. 食品科学, 2006, 27(12): 71-74.

LIU Xiao-yu,HAO Qiang,WU Mou-cheng,YANG Jian,XU Ying. Study on the Determination of Brain AChE Activity in Crucian Carp and Its Sensitivity to Organophosphorus Pesticides[J]. FOOD SCIENCE, 2006, 27(12): 71-74.

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