食品科学 ›› 2006, Vol. 27 ›› Issue (12): 146-148.

• 基础研究 • 上一篇    下一篇

共轭亚油酸高产菌株的诱变选育

于国萍,李庆章,霍贵成   

  1. 东北农业大学食品学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Screening and Breeding of High CLA-Producing Strains by Mutation

YU Guo-ping,LI Qing-zhang,HUO Gui-cheng   

  1. College of Food Science, Northeast Agricultural University, Harbin 150030, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 共轭亚油酸在抗癌、减少脂肪沉积、延缓动脉硬化等方面表现出来功效,推动了共轭亚油酸生产的研究。本研究以寻找到的共轭亚油酸的生成菌株德氏乳杆菌保加利亚亚种为出发菌株,采用紫外线、硫酸二乙酯、亚硝基胍进行多次及复合诱变处理,以提高菌株Ldb2生成共轭亚油酸的能力,获得了315UU、98DD及71UN突变菌株,使共轭亚油酸的生成能力较出发菌株提高幅度在43.04%~59.56%。

关键词: 共轭亚油酸, 德氏乳杆菌保加利亚亚种, 诱变

Abstract: In recent years, CLA has attracted strong interest in food science and madicine because some of the isomers are believed to have several beneficial effects. Anticarcinogenesis, antiarteriosclerosis, inhibition of body fat accumulation, growth and immune stimulation were inclued. Strains mutation happened by using ultraviolet, DNS, NTG, and their complex for promoting CLA level. The new strains of 315UU, 98DD, 1616UU and 71UN were obtained, and the ability of CLA production increased 43.04%~59.56% than before.

Key words: conjugated linoleic acid(CLA), Lactobacillus delbrueckii subsp. bulgaricus, mutation