食品科学 ›› 2006, Vol. 27 ›› Issue (12): 182-185.

• 基础研究 • 上一篇    下一篇

荔枝壳中多酚氧化酶活性研究

熊何健,卢玉兰   

  1. 集美大学生物工程学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Characteristics of Polyphenol Oxidase in Lichi Hull

XIONG He-jian,LU Yu-lan   

  1. School of Bioengineering, Jimei University, Xiamen 361021, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 本文对荔枝壳中多酚氧化酶(PPO)的活性及不同抑制剂的抑制效果进行研究。结果表明:荔枝壳多酚氧化酶的最佳底物浓度为0.04mol/L,最适pH为7.0,最适温度为40℃;柠檬酸、抗坏血酸、硫代硫酸钠对PPO活性表现出较好的抑制效果,三种抑制剂联合作用的最佳条件为0.01mmol/L的柠檬酸+0.04mmol/L的抗坏血酸+0.004mmol/L的硫代硫酸钠,PPO活性的抑制率达85.8%。

关键词: 荔枝壳, 多酚氧化酶, 活性, 抑制

Abstract: The activity of PPO of lichi hull and the control effect for different inhibitor were studied in this paper. The result showed that the fine substrate consistence was 0.04mol/L, the proper pH was 7.0, the optimum temperature was 40℃ for PPO of lichi hull;citric acid, ascorbic acid,hyposulphite display better inhibition effect on PPO activity. The optimum inhibition condition was 0.05mmol/L citric acid add 0.2mmol/L ascorbic acid add 0.02mmol/L hyposulphite, the PPO residual activity was 14.2%.

Key words: lichi hull, polyphenol oxidase, activity, inhibition