UV、LiCl复合诱变深黄被孢霉选育γ-亚麻酸突变株

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 201-203.

UV、LiCl复合诱变深黄被孢霉选育γ-亚麻酸突变株

魏娜，李柏林，欧杰，蒋志斌

上海水产大学食品学院；

出版日期:2006-12-15 发布日期:2011-11-23

Screening of A Producing γ-linolenic Acid Mutant Strain(Mortierella isabellina) by UV and LiCl

WEI Na,LI Bai-lin,OU Jie,JIANG Zhi-bin

College of Food Science, Shanghai Fisheries University, Shanghai 200090, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 以深黄被孢霉(Mortierellaisabellina)AS3.3410为出发菌株,经UV、LiCl复合诱变处理,随机筛选后复筛,最终获得一株突变株SHFU-13。其种子发酵2d,摇瓶发酵8d时,生物量和总脂量分别达21.66、12.65g/L,在同等条件下比出发菌株分别提高了8.84%和36.61%。气相色谱分析其油脂组成,γ-亚麻酸含量为6.39%,产量为0.81g/L,产量较对照样提高44.64%。连续传代多次,其产量性状无显著变化。

关键词: 深黄被孢霉, &gamma, -亚麻酸, 诱变

Abstract: A mutant strain SHFU-13 was successfully obtained when the original strain Mortierella isabellina AS3.3410 was treated by UV and LiCl as mutating agent. The biomass was up to 21.66g/L, which was 8.84% higher than that of the original strain while the product of the total lipid was up to 12.65g/L, which was 36.61% higher than that in the original strain mycelium. Analysis by gas chromatograph indicated the content of GLA in the lipid was 6.39% while the product of it was up to 0.81g/L, which was 44.64% higher than that of the original strain. After passing on many times, there were no distinct changes on the production.

Key words: , Mortierella isabellina； GLA； mutation；

魏娜，李柏林，欧杰，蒋志斌. UV、LiCl复合诱变深黄被孢霉选育γ-亚麻酸突变株[J]. 食品科学, 2006, 27(12): 201-203.

WEI Na,LI Bai-lin,OU Jie,JIANG Zhi-bin. Screening of A Producing γ-linolenic Acid Mutant Strain(Mortierella isabellina) by UV and LiCl[J]. FOOD SCIENCE, 2006, 27(12): 201-203.

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