食品科学 ›› 2007, Vol. 28 ›› Issue (3): 203-205.

• 生物工程 • 上一篇    下一篇

甘油脱水酶结构基因(gldABC)克隆及序列分析

 郑艳, 管艺飞, 刘长江   

  1. 沈阳农业大学食品学院食品生物技术实验室; 辽宁省人民政府 辽宁沈阳110161; 辽宁沈阳110161; 辽宁沈阳110000;
  • 出版日期:2007-03-15 发布日期:2011-12-31

Gene Clone and Sequence Analysis of Glycerol Dehydratase

 ZHENG  Yan, GUAN  Yi-Fei, LIU  Chang-Jiang   

  1. 1.Food Biotechnology Laboratory, College of Food Science, Shenyang Agricultural University, Shenyang 110161, China; 2.The Government of Liaoning Province, Shenyang 110000, China
  • Online:2007-03-15 Published:2011-12-31

摘要: 以肺炎克雷伯氏菌As1.1736的基因组DNA为模板,通过PCR扩增得到了目的基因(gldABC)并将该基因克隆到pMD19-TSimple载体。通过对该基因的序列分析,甘油脱水酶(gldABC)结构基因的全长为2816bp,由三个独立的开放阅读框架组成,三个独立的读码框分别由1668、585、426bp组成,分别编码556、195、142个氨基酸。通过BLAST同源性分析,该基因与GenBank中已发表的gldABC基因(U60992)的核苷酸序列同源性为99.39%。氨基酸的同源性为100%。

关键词: 肺炎克雷伯氏菌, 1, 3-丙二醇, 甘油脱水酶, 克隆

Abstract: The gene of gldABC encoding glycerol dehydratase was amplified by PCR and cloned into the vector of pMD19- T Simple marker. Sequence analysis showed that the gene of gldABC is 2816bp including three open reading frames encoded polypeptides of 556,194 and 142 amino acids respectively. The deduced amino acid sequences homologized with Klebsiella pneumoniae ATCC25955 is 100%.

Key words: K.pneumoniae, 1,3-propanediol, glycerol dehydratase, clone