食品科学 ›› 2007, Vol. 28 ›› Issue (3): 219-222.

• 生物工程 • 上一篇    下一篇

野生型与突变型钝齿棒杆菌生物合成精氨酸基因簇arg JBDFR的生物信息学比较

 陈雪岚, 熊勇华, 陶文沂, 王正祥, 许正宏   

  1. 江西师范大学生命科学学院; 南昌大学中德联合研究院; 江南大学生物工程学院; 江南大学生物工程学院 江西南昌330002; 江西南昌330047; 江苏无锡214036;
  • 出版日期:2007-03-15 发布日期:2011-12-31

Informatics Comparisons of Arginine Biosynthetic argCJBDFR Gene Cluster from C.crenatum A.S 1.542 and C.crenatum A.S.M2

CHEN  Xue-Lan, XIONG  Yong-Hua, TAO  Wen-Yi, WANG  Zheng-Xiang, XU  Zheng-Hong   

  1. 1.College of Life Science, Jiangxi Normal University, Nanchang 330002, China; 2.Sino-Germany Joint Research Insititute, Nanchang University, Nanchang 330047, China; 3.School of Biotechnology,Southern Yangtze University, Wuxi 214036, China
  • Online:2007-03-15 Published:2011-12-31

摘要: 本文设计四对特异性引物分别扩增了野生型和突变型钝齿棒杆菌精氨酸生物合成基因簇argCJBDFR,对其中发生突变的基因argJ、argB及argF的变化进行了生物信息学比较,并分析了这些突变可能对精氨酸产量提高的贡献,为采用基因工程手段改良菌种提供有益的信息和指导。

关键词: 钝齿棒杆菌, 精氨酸, 生物信息学

Abstract: In this paper, four pairs of primers were designed for the arginine biosynthetic argCJBDFR gene clusters amplification from C.crenatum A.S 1.542 and C. crenatum A.S.M2, respectively. The mutation genes:argJ, argB and argF from C.crenatum A.S.M2 were compared with those from C.crenatum A.S 1.542 on bioinformatics, and were analyzed for the possible contribution to enhance the arginine production. These results will offer useful information and instruction to improve strains by gene engineering.

Key words: Corynbacterium crenatum, arginine(Arg), bioinformatics