食品科学 ›› 2007, Vol. 28 ›› Issue (3): 293-296.

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定食品中的单糖、双糖

 刘玉峰, 李黎, 李东, 王晶, 方向, 吴方迪   

  1. 北京市营养源研究所分析室; 国家标准物质研究中心; 国家标准物质研究中心 北京100069; 北京100069; 北京100013;
  • 出版日期:2007-03-15 发布日期:2011-12-31

Determination of Monosaccharide and Disaccharide in Foods by HPLC Method

 LIU  Yu-Feng, LI  Li, LI  Dong, WANG  Jing, FANG  Xiang, WU  Fang-Di   

  1. 1.Laboratory Department, Beijing Research Institute for Nutritional Resources, Beijing 100069, China; 2.National Research Center for Standard Substances, Beijing 100013, China
  • Online:2007-03-15 Published:2011-12-31

摘要: 本文建立了高效液相色谱法定量测定食品中的单糖、双糖,确定的色谱条件如下:WatersNH2氨基色谱柱(250mm×4.6mm),乙腈:水85:15(V/V)为流动相,流速1.0ml/min,示差折光检测器,40℃柱温,进样量10μl,外标法定量。该方法的精密度RSD<5%,回收率大于97%。在此色谱条件下20min内完成了单糖、双糖的分离,结果比较理想。研究表明此新建方法准确而快速,并能适用于多数食品中单糖、双糖的测定。

关键词: 高效液相色谱, 单糖、双糖, 食品

Abstract: HPLC method was established to determine the monosaccharide and disaccharide in foods. The chromatographic conditions are as belows: Waters amino chromatographic column with refractive index detector, elution influent methane:water (V/V) 85:15, flow rate 1.0ml/min and injection volume 10μl. The repeatability relative standard deviation of this method is below 5% and the recovery is over 97%. A satisfsacfory result is obtained under these optimum conditions, and the whole separation can be finished with in 20min. The results showed that this new HPLC analysis method is both correct and fast enough to be used in most kinds of foods contained monosaccharide and disaccharide.

Key words: HPLC, monosaccharide and disaccharide, foods