食品科学 ›› 2007, Vol. 28 ›› Issue (4): 125-128.

• 工艺技术 • 上一篇    下一篇

酵母菌发酵玉米皮酸水解液制备结晶L-阿拉伯糖的研究

 李道义, 闫巧娟, 江正强, 石波, 李里特   

  1. 中国农业大学食品科学与营养工程学院; 中国农业大学工学院; 中国农业科学院饲料研究所; 中国农业大学食品科学与营养工程学院 北京 100083; 北京 100083; 北京 100081;
  • 出版日期:2007-04-15 发布日期:2011-12-31

Preparation of Crystalline L-arabinose from Corn Seed-coat Acid Hydrolysis Fermented by Specific Yeast

 LI  Dao-Yi, YAN  Qiao-Juan, JIANG  Zheng-Qiang, SHI  Bo, LI  Li-Te   

  1. 1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China; 2.College of Engineering,China Agricultural University,Beijing 100083,China; 3.Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2007-04-15 Published:2011-12-31

摘要: 玉米皮是玉米淀粉工业的主要副产品,含有大量的阿拉伯木聚糖。本文研究了酵母菌发酵玉米皮酸水解液制备结晶L-阿拉伯糖的方法。玉米皮经稀硫酸水解后得到了以L-阿拉伯糖、木糖和葡萄糖为主要成分的水解液,碳酸钙中和后直接利用酵母菌WYS15-3发酵特异性除去糖液中的木糖和葡萄糖,再通过活性炭脱色、离子交换树脂脱离子、浓缩结晶等步骤,得到了L-阿拉伯糖晶体,得率为9.6%(以玉米皮干基计)。该法简单易行,适用于以玉米皮为原料工业化制各L-阿拉伯糖。

关键词: L-阿拉伯糖, 玉米皮, 酵母菌, 制备, 结晶

Abstract: Corn seed-coat is a major byproduct of the wet-milling cornstarch industry and rich in arabinoxylan.The method of L-arabinose crystalline preparation was carried out in the report.After corn hull being hydrolyzed by the diluted sulfuric acid, the hydrolysis solution was found mainly consisting of arabinose,xylose and glucose.Yeast WYSI5-3 was aerobically cultured in the hydrolyzed solution followed by neutralizing with calcium carbonate to remove xylose and glucose.The result solution was decoiorized with active carbon,desalted with exchange resins and then concentrated.L-arabinose is obtained as crystalline state from aqueous ethanol,and the yield is 9.6% based on corn seed-coat dry mass.The method is easy,feasible,and suitable for industrial preparation of L-arabinose from corn seed-coat.

Key words: L-arabinose, corn seed-coat, yeast, preparation, crystalline