猪血中过氧化氢酶提取及其性质研究

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 311-314.

猪血中过氧化氢酶提取及其性质研究

田荟琳，张坤生

天津商学院生物技术与食品科学学院；

出版日期:2006-12-15 发布日期:2011-11-23

Study on Extraction of Hydrogen Peroxidase from Pig Blood and Its Propertities

TIAN Hui-lin,ZHANG Kun-sheng

School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： As one kind of oxidases, catalases can be found in almost all animals, plants and microorganisms. Catalases can catalyse the decomposition of the cellular hydrogen-peroxide and thus protect membrane lipid against peroxidation. A catalase from pig blood has been purified by the use of sephadex G-100 and PEG-6000 chromatography. It has biggest activity at pH7.4, and 20～ 40℃. There were different effection for different metal ions.

关键词: 过氧化氢酶, 猪血, 提取

Abstract: As one kind of oxidases, catalases can be found in almost all animals, plants and microorganisms. Catalases can catalyse the decomposition of the cellular hydrogen-peroxide and thus protect membrane lipid against peroxidation. A catalase from pig blood has been purified by the use of sephadex G-100 and PEG-6000 chromatography. It has biggest activity at pH7.4, and 20～ 40℃. There were different effection for different metal ions.

Key words: hydrogen peroxidase(catalase), pig blood, extraction

田荟琳，张坤生. 猪血中过氧化氢酶提取及其性质研究[J]. 食品科学, 2006, 27(12): 311-314.

TIAN Hui-lin,ZHANG Kun-sheng. Study on Extraction of Hydrogen Peroxidase from Pig Blood and Its Propertities[J]. FOOD SCIENCE, 2006, 27(12): 311-314.

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