食品科学 ›› 2007, Vol. 28 ›› Issue (4): 217-219.

• 生物工程 • 上一篇    下一篇

酶法提取、超滤分离香菇多糖新工艺研究

 谢红旗, 周春山, 杜邵龙, 周尽花   

  1. 中南大学化学化工学院; 中南大学化学化工学院 湖南 长沙 410083; 湖南 长沙 410083;
  • 出版日期:2007-04-15 发布日期:2011-12-31

Study on Enzymatic Extraction and Ultrafiltration Separation of Lentinan

 XIE  Hong-Qi, ZHOU  Chun-Shan, DU  Shao-Long, ZHOU  Jin-Hua   

  1. College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China
  • Online:2007-04-15 Published:2011-12-31

摘要: 采用中性蛋白酶辅助水提法提取香菇多糖,利用超滤膜分离多糖,研究了酶法提取条件和超滤膜及分离参数的选择。结果表明,中性蛋白酶在pH4.8,温度50℃,处理时间60min条件下,能有效提高多糖的提取率,降低杂质蛋白质的含量。采用截留分子量为50、300kD陶瓷超滤膜,在温度35℃,压力0.15MPa的中性环境下,能有效浓缩香菇多糖提取液,并将多糖分级为三部分:不含蛋白质的小分子量多糖、含少量蛋白质的中等分子量多糖和含大量蛋白质的大分子多糖。

关键词: 香菇多糖, 中性蛋白酶, 酶处理, 超滤, 分级

Abstract: A new lentinan extracting technology from lentinus edodes was developed with neutral proteinase,and the separation of lentinan by microfiltration was studied as well.The crude powder of lentinus edodes was treated by neutral proteinase before extraction in water.The optimized enzyme-based extraction conditions are as follows:treatment temperature 50℃,pH value of extraction media 5.0,extraction time 60 min and mass ratio of enzyme to lentinus edodes 1.5:100.Comparing with traditional extracting technology by water,the extraction yield of lentinan increases 40%,and the concentration of protein decreases 50% with the enzyme-based extracting technology.With two kinds membrane molecular weight cut-off 50 kD and 300 kD,the lentinan is separated into three parts:the small molecular weight polysaccharides without protein,the middle molecular weight polysaccharides with a tittle protein,and the large molecular weight polysaccharides with large amount of protein.The optimized separation conditions of ultrafiltration are as follows:temperature 35℃,pH value 7 and pressure 0.15MPa.

Key words: lentinan, neutral proteinase, enzyme treatment, ultrafiltration, classify