食品科学 ›› 2003, Vol. 24 ›› Issue (4): 117-118.

• 分析检测 • 上一篇    下一篇

毛细管区带电泳法测定绿茶中绿原酸的含量

 张荷丽, 颜振宁   

  1. 郑州牧业工程高等专科学校,郑州大学化学系
  • 出版日期:2003-04-15 发布日期:2011-12-13

Determination of Chlorogenic Acid in Green Tea by CZE

 ZHANG  He-Li, YAN  Zhen-Ning   

  • Online:2003-04-15 Published:2011-12-13

摘要: 应用毛细管区带电泳法测定绿茶中绿原酸的含量。运行缓冲液为pH=8.520mmol/L的硼砂体系;分离电压为20kV,分离温度为20℃;用50%甲醇提取,以苯甲酸钠为内标物,在328nm下进行测定。测得绿茶中绿原酸的含量为10.25mg/g。线性范围是0.03~0.15mg/ml,平均回收率是99.75%,RSD是1.4%。

关键词: 毛细管电泳, 绿茶, 绿原酸

Abstract: Capillary zone electrophoresis (CZE) was used to determine t he cont ent of chlorogenic acid in green tea . The buffer solution used in this method was pH 8.5 and 20 mmol/L sodium borate solution .Under 20℃, the separating vol tage was 20kV . Sodium benzoate used as interna l standard and 50% methanol use d as extraction , the wavelength of de tection was 328 nm .The content of chloro genic acid was 10.25 mg/g in g reen tea , the calibration curve showed good lin earity over the range of 0.03~0.15 mg/ml, the average recovery was 99.75% , RSD was 1.4% .

Key words: CZE, green tea, chlorogenic acid