关键词: 蟾蜍, 氨基酸, 多不饱和脂肪酸, 单不饱和脂肪酸

Abstract: Using HITACHI L-8800 auto amino acid analyzer instrument and SHIMADZU LC-6A high performance liquid chromatography to determine the content of amino acid and fatty acid in five fractions of Bufo, and all the results was deal with SPSS12.5. The results showed that the contents of amino acid in Bufo liver and skin have significant difference (p<0.05); the contents of amino acid in Bufo liver, egg and skin have very significant difference (p<0.01) as well as the contents of amino acid in bufo bone, egg, meat and skin, the contents of amino acid in Bufo egg and meat; the contents of fatty acid between Bufo liver and egg, between Bufo bone and egg, between Bufo bone and skin, between Bufo egg and skin, between Bufo egg and meat have very very significant difference (p<0.01); but the contents of fatty acid between Bufo bone and liver, between Bufo meat and liver, between Bufo skin and liver, between Bufo meat and bone, between Bufo meat and skin have no significant difference(p> 0.05). It showed that the Bufo egg is rich in polyunsaturated fatty acid (MUFA) and monousaturated fatty acid(PUFA).

Key words: Bufo, amino acid, PUFA, MUFA