食品科学 ›› 2007, Vol. 28 ›› Issue (4): 276-279.

• 分析检测 • 上一篇    下一篇

苯酚-硫酸法测定野木瓜中多糖含量的研究

 王文平, 郭祀远, 李琳, 王明力, 梁桂娟   

  1. 华南理工大学轻工与食品学院; 贵州省发酵工程与生物制药重点实验室; 贵州省发酵工程与生物制药重点实验室 广东 广州 510640; 广东 广州 510640; 贵州 贵阳 550003;
  • 出版日期:2007-04-15 发布日期:2011-12-31

Assay Study on Content of Polysaccharides in Stanuntonia Chinensis by Phenol-sulfuric Acid Method

 WANG  Wen-Ping, GUO  Si-Yuan, LI  Lin, WANG  Ming-Li, LIANG  Gui-Juan   

  1. 1.College of Light Industry and Food Science,South China University of Technology,Guangzhou 510640,China; 2.Guizhou Province Key Labaratory of Fermentation Engineering and Biopharmary,Guiyang 550003,China
  • Online:2007-04-15 Published:2011-12-31

摘要: 采用苯酚-硫酸法对野木瓜中的多糖含量进行测定,在波长490nm处测定吸光度,实验表明标准葡萄糖在8~64μg之间呈良好的线性关系,该法简便易行,准确性高,重现性好。本文探讨了测定过程的影响因素,并通过此法测得野木瓜中多糖含量为12.02%。

关键词: 野木瓜, 多糖, 苯酚-硫酸法, 含量

Abstract:  The content of polysaccharides in Stanuntonia Chinensis was determined by the method of phenol-sulfuric acid,to assay the absorbance at 490 nm.The results showed that there is a good linearity relationship between the absorbance and the content of standard glucose in the range of 8~64μg.The method is simple,convenient and accurate,and has good reproducibility. The affecting factors in the assaying process were discussed in this paper respectively.By this method,the content of polysaccharides in Stanuntonia Chinensis is found to be 12.02%.

Key words: Stanuntonia Chinensis, polysaccharides, phenol-sulfuric acid method, content