食品科学 ›› 2003, Vol. 24 ›› Issue (4): 38-41.

• 基础研究 • 上一篇    下一篇

壳聚糖膜固定化葡萄糖氧化酶的特性研究

 姜梅, 王善荣, 季勤   

  1. 南京农业大学食品科技学院
  • 出版日期:2003-04-15 发布日期:2011-12-13

Study on Properties of Immobilized Glucose Oxidase on Chitosan Membrane

 JIANG  Mei, WANG  Shan-Rong, JI  Qin   

  • Online:2003-04-15 Published:2011-12-13

摘要: 研究了以壳聚糖膜为载体、戊二醛为交联剂的固定化葡萄糖氧化酶的特性。结果表明:固定化酶、溶液酶的最适pH均为5.5;固定化酶、溶液酶的最适温度分别为40℃,35℃;固定化酶的Km值为12.16mmol/L,溶液酶的Km值为20.86mmol/L;固定化酶比溶液酶更耐热,且贮藏稳定性及操作稳定性也有所提高;该固定化酶重复使用率较高。

关键词: 葡萄糖氧化酶, 固定化酶, 壳聚糖膜, 特性

Abstract: The propertie of glucose oxidase covalently immobilized on glutarald e h yde-treated chitosan membrane change.The optimum pH value for Immobilized glu co se oxidase (IGOD) and glucose oxidase(GOD)are 5.5;The optimum temperature fo r I GOD and GOD were 40℃ and 35℃,respectively. The apparent Km of IGOD is 12. 06 mmol/L while Km of GOD is 20.86mmol/L:the thermal stability of IGOD is im prov ed by immobilizati on. The immobilized enzyme had good storage and operatio n st ability and reusability and that properties offer potential for further pra ctica l application.

Key words: Glucose Oxidase, immobilized enzyme, chitosan membrane, properties