食品科学 ›› 2003, Vol. 24 ›› Issue (4): 19-22.
• 基础研究 • 下一篇
杨宏顺, 陈复生, 李云飞, 刘芳
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YANG Hong-Shun, CHEN Fu-Sheng, LI Yun-Fei, LIU Fang
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摘要： 系统地研究了碱性蛋白酶2709在AOT/异辛烷反胶束体系中的催化行为。研究底物浓度、反胶束浓度、水含量、pH值、温度、盐的浓度及种类对酶活力的影响。结果表明:酶在反胶束体系中表现出超活性,且作用于胶束膜。在AOT/异辛烷浓度为0.08g/ml时,影响酶活力大小主次因素为:盐浓度>W0>pH值>温度。最佳酶活力条件:45℃,0.2mol/LKCl液,pH值9.2,W值19.3。此时酶活力为9.27×105U/g。
关键词: 反胶束, 碱性蛋白酶, 酶活力
Abstract: The enzyme activity of alkalase 2709 incorporated in AOT/iso-octane reverse micelles has been investigated. Effects of substrate concentration, rev erse micelles concentration, W0, pH, temperature, salt concentration and of vari ous kinds were studied in detail. The enzyme showed "super activity" and acted i n the membrane of reverse micelles. When the concentration of AOT/iso-octane wa s 0.08g/ml, factors affecting the activity of the enzyme were as follows: sal t concentration > W0 >pH value >temperature with optimal activity at 9.27×105U ,4 5℃,0.2mol/L KCl, pH 9.2 andW0 value 19.3.
Key words: reverse micelles, alkalase 2709, enzyme activity
杨宏顺, 陈复生, 李云飞, 刘芳. 反胶束中碱性蛋白酶2709的活力研究[J]. 食品科学, 2003, 24(4): 19-22.
YANG Hong-Shun, CHEN Fu-Sheng, LI Yun-Fei, LIU Fang. Enzyme Activity Study on Alkalase 2709 in Reverse Micelles[J]. FOOD SCIENCE, 2003, 24(4): 19-22.
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