食品科学 ›› 2007, Vol. 28 ›› Issue (4): 289-291.

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定食品中的胆固醇

 李黎, 刘玉峰, 唐华澄, 李东, 王晶, 方向, 吴方迪   

  1. 北京市营养源研究所分析室; 国家标准物质研究中心; 国家标准物质研究中心 北京 100069; 北京 100069; 北京 100013;
  • 出版日期:2007-04-15 发布日期:2011-12-31

Determination of Cholesterol in Foods by HPLC Method

 LI  Li, LIU  Yu-Feng, TANG  Hua-Cheng, LI  Dong, WANG  Jing, FANG  Xiang, WU  Fang-Di   

  1. 1.Laboratory Department of Beijing Research Institute for Nutritional Resources,Beijing 100069,China; 2.National Research Center for Standard Substances,Beijing 100013,China
  • Online:2007-04-15 Published:2011-12-31

摘要: 本文建立了高效液相色谱法测定动物性食品中胆固醇的含量,确定的色谱条件如下:C18色谱柱,二极管阵列检测器,205nm检测波长,100%甲醇作为流动相,1.0ml/min流速。该方法的精密度RSD小于5%,回收率大于96%~102%。在此色谱条件下15min内完成了胆固醇的分离,结果比较理想。研究表明此新建方法准确而快速,并能适用于绝大部分食品中胆固醇的测定。

关键词:  , 高效液相色谱, 胆固醇, 食品

Abstract: HPLC method was established to determine cholesterol in animal foods.The final chromatographic conditions are:C18chromatographic column,diode-array detector,detector wave 205 nm,100% methane elution influent and flow rate 1.0ml/min.The relative standard deviations of this method is below 5% and the recovery is between 96%~102%.The satisfied results can be obtained under this chromatographic conditions,and the separation can be finished within 15 min.The results showed this new HPLC analysis method is both correct and fast enough to be used in assaying most foods containing cholesterol.

Key words: HPLC, cholesterol, food