大豆甙元抑制去卵巢大鼠骨矿丢失和相关元素关系的研究

食品科学 ›› 2007, Vol. 28 ›› Issue (4): 317-320.

大豆甙元抑制去卵巢大鼠骨矿丢失和相关元素关系的研究

吕伶, 金邦荃, 周耀民, 詹巍

南京市妇幼保健院；南京市师范大学；南京市师范大学江苏南京 210004；江苏南京 210097；

出版日期:2007-04-15 发布日期:2011-12-31

Effects of Daidzein on the Relationship among Ca,P,Zn,Mg,Mn and Bone Loss in Ovariectomized Female Rats

吕Ling , JIN Bang-Quan, ZHOU Yao-Min, ZHAN Wei

1.Nanjing Maternal and Child Health Hospital,Nanjing 210004,China; 2.Nanjing Normal University,Nanjing 210097,China

Online:2007-04-15 Published:2011-12-31

摘要/Abstract

摘要： 目的:研究大豆甙元对去卵巢引起的骨质疏松大鼠骨矿物质和相关元素含量的影响。方法:实验用40只三月龄雌性清洁级大鼠,分为4组。其中去卵巢+己烯雌酚组(OVX-E)、去卵巢+大豆甙元组(OVX-D)大鼠每日分别灌胃己烯雌酚或大豆甙元,另二组大鼠仅灌胃等量蒸馏水。饲喂三个月后(90d)处死,收集血和骨样,分别测定钙(Ca)、磷(P)、锌(Zn)、镁(Mg)、锰(Mn)和碱性磷酸酶(ALP)等指标。结果去卵巢后雌性大鼠血清Ca、P、Zn、Mg含量无明显变化,但灌胃己烯雌酚或大豆甙元的去卵巢大鼠血清P、Zn、Mg含量则较假手术组(Sham)和去卵巢组(OVX)大鼠显著降低(p＜0．01,p＜0．05)。与OVX组相比,OVX-E和OVX-D组大鼠骨骼Ca、Zn含量得到改善;血清和胫骨钙磷比(Ca:P)的显著升高,分别为增加36．4%和48．6%(p＜0．01),接近Sham组。结论:研究进一步证实大豆甙元在改善骨密度的机理之一是,通过减少血清P、Zn、Mg吸收,提高Ca的吸收利用率,有利于Ca和Zn在胫骨中沉积,而增强骨密度。但血清碱性磷酸酶的表达主要与内源性雌激素水平有关。

关键词: 大豆甙元, 钙, 锌, 去卵巢大鼠

Abstract: Objective:This study was designed to research the effects of daidzein on bone calcium metabolism in ovariectomized rats.Methods:Forty 90-day old female Sprague Dawley rats were randomly divided into four groups.10 rats were operated with ovary as Sham group for the control（Sham）.Other 30 rats were ovariectomized for OVX,OVX-E（Drenched 100μg/kg·d bw. d Diethylstilbestrol,DES）and OVX-D group（Drenched 100mg/kg·d bw Daidzein,Da）,10 in each one.After feeding 90 days, all of rats were killed to collect the blood and bone samples.The content of Ca,P,Mg,Zn,Mn in serum and bone were assayed. Results The results showed that the content of Ca,P,Mg,Zn in serum in OVX group were similar to those in Sham group,while P,Mg,Zn were decreased significantly in OVX-E and OVX-D groups compared to Sham groups（p＜0.01,p＜0.05）.The content of bone Ca and Zn in OVX-E and OVX-D groups were increased,compared with OVX group,accessing the normal lever.And then the ratio of Ca:P in serum and bone was increased by 36.4% or 48.6%(p＜0.01**).Conclusion:The Ca utilization and deposition in bone were increased because the higher ratio of Ca:P and lower level of P,Mg,Zn in serum were controlled by Da or DES.However,ALP in serum has relation with endogenous estrogen.

Key words: daidzein, Ca, Zn, ovariectomized female rats

吕伶, 金邦荃, 周耀民, 詹巍. 大豆甙元抑制去卵巢大鼠骨矿丢失和相关元素关系的研究[J]. 食品科学, 2007, 28(4): 317-320.

吕Ling , JIN Bang-Quan, ZHOU Yao-Min, ZHAN Wei. Effects of Daidzein on the Relationship among Ca,P,Zn,Mg,Mn and Bone Loss in Ovariectomized Female Rats[J]. FOOD SCIENCE, 2007, 28(4): 317-320.

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