食品科学 ›› 2003, Vol. 24 ›› Issue (3): 119-122.

• 营养卫生 • 上一篇    下一篇

复合蜂胶制剂调节肠道功能的体外实验研究

 王南舟   

  1. 杭州商学院食品科学与工程系
  • 出版日期:2003-03-15 发布日期:2011-12-13

Study on Myenteric Motion Tests in Vitro by Compound Propolis Formulae

 WANG  Man-Zhou   

  • Online:2003-03-15 Published:2011-12-13

摘要: 本文利用蜂胶乙醇提取液与中药芍药甘草汤配伍制成复合蜂胶制剂。以此为样品,进行离体肠肌运动试验,并以福氏痢疾杆菌、沙门氏菌、金黄色葡萄球菌、大肠杆菌、绿脓杆菌、变形杆菌等肠道致病菌和条件致病菌为对象,进行体外抑菌试验。实验结果表明:本制剂对小肠平滑肌蠕动具有一定的抑制作用和明显的解痉镇痛作用,通过本制剂与神经递质乙酰胆碱、肾上腺素和受体阻断剂阿托品对小肠平滑肌的作用相比较,显示本制剂对小肠平滑肌运动的上述影响不是通过α受体、β2受体和M受体来实现的,可能是肌源性的或其它途径。体外抑菌试验的结果显示:本制剂对上述各菌株均有较强的抑制作用,其最低抑菌浓度(MIC)均小于500×10-6,其中对沙门氏菌、福氏痢疾杆菌的MIC为250×10-6。本试验为本制剂作为调理消化功能的功能性食品的开发利用,提供了实验依据。

关键词: 蜂胶, 解痉试验, 抑菌试验, 功能性食品

Abstract: In this study,compound propolis formulae were prepared from making up th e propolis’ ethanol extract with Chinese herbal broths o f licorice(Radix Glycyrrhizac)roots and herbaceous peony(Paeonialaciflora)roots.By means of the formula,myent eric motion tests were then carried o ut in vitro,In vitro bacteriostatic tests were als o conducted against some enteropath ogens and opportunistic pathogens,such as Shigella flexneri,Salmonella,Staphlococcus aureus,Escherichia coli,Pseudomonas aeruginosa and Proteus.The tests revealed that the formula showed certain inhibitive effect on the peristalsis of in-testinal smooth muscles and also rem arkable antispasmodic and abirrita nt effects.Compared with acetylcholine(Ach)and epinephrine,two neutrotransmi tters and atropine,an antagonist,t he actions of compoundpropolis formula on intestinal smooth muscles have not realize dthroughα-,β-or M-re-ceptors.Instead,they were probabl ymyogenicorviaotherways.Inthein vitrobacteriostatictests,theformula showedstronginhibitiveeffecn on t he bacterial strains tested.All of t heir MICs were lower than500×10 -6 ,while those on Salmonella and Shigella flexneri were both250×10 -6 .The study formed a pharmacological experimental basis for the application of compound propolis formulatio ns as a functional food to regulate digestivefunctions.

Key words: propolis, antispasmodic test, bacte riostatic tests, functional food