食品科学 ›› 2003, Vol. 24 ›› Issue (2): 29-32.

• 基础研究 • 上一篇    下一篇

脱苦大豆多肽产生菌的筛选及其水解条件的优化

 万琦, 陆兆新, 高宏   

  1. 南京农业大学食品科技学院
  • 出版日期:2003-02-15 发布日期:2011-12-13

Study on Screening of a Bacillus Subtilis Strain to Produce Debittering Soybean Peptides and Optimizing Its Liquid Fermention Conditions

 WAN  Qi, LU  Zhao-Xin, GAO  Hong   

  • Online:2003-02-15 Published:2011-12-13

摘要: 从八株枯草芽孢杆菌(Bacillussubtilis)中筛选出了一株适合于豆粕粉水解的B.subtilis菌株;通过正交试验确定最佳水解条件为:豆粕粉6%,pH6.0,温度30℃,发酵时间48h;用某曲霉菌产生的羧肽酶水解B.subtilis的发酵液,经氨基酸自动分析仪测定产物中游离氨基酸的含量和种类,初步确定了B.subtilis蛋白酶的主要酶切位点。

关键词: 枯草芽孢杆菌, 大豆多肽, 羧肽酶

Abstract: One strain, being fit for the hydrolysis of defatted soybean, was screened out from eight Bacillus subtilis strains. Through the orthogonal test, the optimum conditions of hydrolysis were ascertained: 6%defatted soybean, natural pH tempreture 30℃,and the time of fermentation 48h. After analyzing the free amino acids of B.subtilis broth hydrolyzed by one kind of carboxypeptidase from an Aspergillus strain, some specific sites of the proteases were found.

Key words: Bacillus subtilis, soybean peptide, carboxypeptidase