食品科学 ›› 2003, Vol. 24 ›› Issue (1): 112-114.
• 分析检测 • 上一篇 下一篇
杜薇, 刘国文
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DU Wei, LIU Guo-Wen
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摘要: 目的:测定刺梨中总黄酮的含量,为进一步天发利用贵州丰富的刺梨资源提供科学依据。方法:正交设计优选刺梨中总黄酮的提取条件,用芦丁作对照品、三氯化铝作显色剂、测定波长在420nm、比色法测定刺梨中总黄酮含量。结果:优选提取条件是:用65%乙醇提取4h、每次提取体积100ml、提取一次。测定刺梨中总黄酮的含量,1、2、3号样分别为0.773%、0.874%、0.893%。结论:优选提取方法效率高,含量测定方法操作简便,准确,稳定性好。
关键词: 刺梨, 总黄酮, 正交设计, 含量测定
Abstract: Object:To provide scientific basis for further developing and using ric h Cili resources in Guizhou.Methods:The content of total flavon oids was measured,using rutin as con trol and aluminumtrichloride as colori-metricreagentandcolorimetricass ayat420nm.Result:Theoptimumextr actionconditions were:toextract4hwith65%C 2 H 5 OH?with extract volume 100ml everytime .The content of total flavonoid of1?2and 3were 0.773%?0.874%and0.893%respectively.Con dition:Theoptimumextractionmeth odwas efficient.Thedeteminationo f the flavonoid content was simple,steady,accurate and reproducible.
Key words: Cili, total flavonoids, orthogonal design test, determination
杜薇, 刘国文. 刺梨总黄酮的含量测定及资源利用[J]. 食品科学, 2003, 24(1): 112-114.
DU Wei, LIU Guo-Wen. Determination of Content of Total Flavonoids in Cili and Resources Development Prospect[J]. FOOD SCIENCE, 2003, 24(1): 112-114.
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