关键词: 羊胎水解液, 乙醚萃取物, 甾醇, 脂肪酸, 雌二醇

Abstract: The extracts from enzymolysis liquid of sheep placenta ware extracted by diethyl ether and then saponated in KOH-CH3CH2OH.extracted again by diethyl ether, and finally passed a mini-silica gel chromatography column. The elutions were derived by BSTFA and analysed on GC-MC.The results show at that the extracts from enzymolysis liquid of sheep placenta consisted of mainly mainly acids. cholesterol and a small amount of female hormones the C 16∶0.C 18∶0. and C 18∶1△9 acids were dominant in fatty acid series .The amount of cholesterol was highest both in sterol series and in extracts. The single ion m/z 416 and the characteristics of mass spectrum demonstrated that the estradiol existed in extracts .but in quite small amount.

Key words: Enzymolysis lipuid of sheep placenta, Extracts by diethylether, fatty acids, sterols cholesterol, hormone GC-MS analysis