食品科学 ›› 2000, Vol. 21 ›› Issue (1): 11-13.

• 基础研究 • 上一篇    下一篇

南果梨多酚氧化酶最适作用酶促褐变底物的分析确定

马岩松,车芙蓉,张平,周亚凤   

  1. 沈阳农业大学食品科学系
  • 出版日期:2000-01-15 发布日期:2011-11-25

 MA  Yan-Song, CHE  Fu-Rong, ZHANG  Ping, ZHOU  Ya-Feng   

  • Online:2000-01-15 Published:2011-11-25

摘要: 南果梨采后贮藏期间果心、果肉的酚类物质含量呈先合成上升后分解降低趋势,果实出现褐变时期与组织内酚类物质明显减少时期呈现对应关系。同期多酚氧化酶(PPO)活性也相应出现明显高峰。因此说明南果梨贮藏褐变是酚类物质在有氧条件下被PPO催化而造成的酶促褐变。试验分析看到,绿原酸在南果梨褐变前后发生明显量的变化,并能与PPO互相作用形成褐变物质,初步认为绿原酸是南果梨褐变底物酚类物质的主要成分之一。

关键词: 南果梨, 酚类物质, 绿原酸

Abstract: During the postharvest storage of Nanguo pear,the content of fruit core and flesh phenolic substance first increased by composing and then decreased by decomposing,and the period of fruit browning corresponded with the period of phenolic substance decreasing. During the same period,polyphenolic oxidase activity reached its peak.So the storage browning of Nanguo Pear was due to that the phenolic substance was catalyzed by polyphenolic oxidase while oxygen existed. Experimental analyses showed, that the content of chlorogenic acid changed significantly after browning. producing brown substance by interacting with polyphenolic oxidase,so chlorogenic acid was identified as one of the important enzyme brown- ing substrates of Nanguo Pear

Key words: Nanguo Pear, phenolic substance, chlorogenic acid