食品科学 ›› 2000, Vol. 21 ›› Issue (7): 31-34.

• 营养卫生 • 上一篇    下一篇

六月霜提取物清除和OH·自由基的体外实验研究

 张虹, 许钢, 张辉, 张大中, 李新亮   

  1. 杭州商学院食品科学与工程系
  • 出版日期:2000-07-15 发布日期:2011-12-02

In Vitro Studies of Scavenging Capacity of Artemisia anomala S. Moore on Superoxide Free Radical and Hydroxyl Free Radical

 ZHANG  Hong, XU  Gang, ZHANG  Hui, ZHANG  Da-Zhong, LI  Xin-Liang   

  • Online:2000-07-15 Published:2011-12-02

摘要: 用氮蓝四唑(NBT)光还原法,对六月霜中提取物清除超氧离子自由基O-·2的效果进行了测定。结果显示,六月霜提取物对超氧离子自由基有较强的清除效果,清除效果与提取物中黄酮浓度有关,当黄酮浓度达到一定值时,对O-·2的清除效果可高达88.41%。提取物与抗坏血酸(VC)进行了对照实验,结果显示其对O-·2的清除能力比VC高。六月霜提取物在VC-CU2+-H2O2体系中,对OH·自由基的清除效果的研究显示,最高清除率为84.02%。

关键词: 六月霜, 提取物, 清除, 超氧离子自由基■OH·, 自由基

Abstract: The superoxide free radical (o)scavenging capacity of Artemisia anomala S.Moore was studied by nitro blue telrazolium (NBT) photo-ueduction.The results showed that the extract from Artemisia anomala S.Moore had strong scavenging effect on superoxide free radical.The seavenging effect was related to the content of the flavonoids.When the concentration of the flavonoids in extract is high enough it had strong scavenging rate as 88.41 % .Using the models of VCCu2+-H2O2 for hydroxyl radical (OH· ).the scavenging capacity of the extract of Artemisia anomala S.Moore on OH· was investigated.The maximum scavenging capacity on hydroxyl radical was 84.02%.

Key words: Artemisia anomala.S.Moore Extract Scavenge Superoxide free radical Hydroxyl free radical