食品科学 ›› 2000, Vol. 21 ›› Issue (10): 50-52.

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定食品中糖精钠和天冬糖

 张平, 谭慧   

  1. 武汉市卫生防疫站
  • 出版日期:2000-10-15 发布日期:2011-12-02

Determination of Saccharin and Aspartame in food by High Performance Liquid Chromatography(HPLC)

 ZHANG  Ping, TAN  Hui   

  • Online:2000-10-15 Published:2011-12-02

摘要: 利用高效液相色谱测定食品和复合甜味剂蛋白糖中人工合成甜味剂-糖精钠和天冬糖,采用国产C18不锈钢柱(SuperisorbC185μm,70mm×4.6mmi.d)以甲醇-0.02mol/L磷酸缓冲液pH45(30/70)为流动相,于210nm波长下进行检测,采用该方法糖精钠和天冬糖的回收率分别为96.2%~102.7%和99.2%~105.1%;最小检出限分别为0、6ng和1.4ng

关键词: 高效液相色谱法, 糖, 糖精钠, 天冬糖

Abstract: A HPLC procedure for the assay of combined sweetening Saccharin and Aspartame in food with the column packed with 5μ m Spherisorb C18 was presented.The methanol and 0.02mol/L phosphate buffer pH4.5 (30.70) mixture was used as mobile phase and .the UV detector wavelength was 210nm.The recoveries of Saccharin and Aspartame were 96.29%~102.7% and 99.2%~105. 1 % respectively,.The determination limits of Saccharin and Aspartame were 0.6ngand 1 .4ng respectively.

Key words: HPLC Saccharin Aspartame