关键词: 发酵液, 酵母浓度, 实明在线检测, 电容率分布, 高温灭菌

Abstract: This paper concerns a device and a method which monitor ydast concentrations on-line and in-situ in a fermentation process The presence of microbial cells would affect the dielectrical properties of microbial suspensions .At radio -frequencies the intrinsic passive electrical properties of cell suspensions are completely specified by its conductivity and permittivity,At radio -frequencies the dielectric permittivity of cell suspensions are monotonic function of the measuring frequency and cell density. Based on this understanding a new method for measuringbiomass concentration is proposed .Using this method the biomass concentrations are measured on-line and in-situ without taking samples from the bioreactor, and only viable cells are detected.The electrode which we developed could be directly inserted into the fermentor and could be sterilized in place.The method has wide use in the industrial field of pharmacy. brewery, sewage disposal.

Key words: Cell suspension, Yeast density, On-line and in-situ monitoring, Permittivity, Sterilizing in place