食品科学 ›› 2002, Vol. 23 ›› Issue (9): 91-93.

• 分析检测 • 上一篇    下一篇

OPA-FMOC柱前衍生反相液相色谱法测定氨基酸含量

 叶英   

  1. 农业部食品质量监督检验测试中心
  • 出版日期:2002-09-15 发布日期:2011-12-31

Determine Amino Acid Contents with OPA-FMOC Pre-column Derivatization RP-HPLC

 YE  Ying   

  • Online:2002-09-15 Published:2011-12-31

摘要: 采用邻苯二甲醛一氯甲酸芴甲酯(OPA-FMOC)柱前衍生反相高效液相色谱法测定样品中氨基酸含量,以HypersilC18为分析柱,采用荧光检测器,17min内16种氨基酸完全分离。分离效果,重现性,线性范围均令人满意。

关键词: 反相高效液相色谱法, 柱前衍生, 氨基酸

Abstract: A method using pre-column derivatization with o-phthaldialdehyde(OPA) and fluorenylmethyl chloroformate(FOMC chloride)was reported for the determination of amino acids in sample by RP-HPLC.The analysis could be accomplished within 17.0 min by using Hypersil C18. RP-HPLC followed by FLD detection. The method possesses the advantages of effective separation, good reproducibility and satisfactory linear range.

Key words: RP-HPLC Pre-column , Derivatization , Amino acid