关键词: 盐藻, &beta, －胡萝卜素, 二步培养法

Abstract:  The Dunaliella salina cultured in the optimum growth condition for 4 ~5days was gradually transferred in the optimum condition of β-carotene accumulation. The results showed that the yield of β-carotene of the two stage culture was 46%higher than that of the direct culture in the optimum output condition.The output of β carotene .by the two stage culture outdoors,Was 38.5mg/L,above one time higher than that of by the extensive high density culture .The results explained that the application of two stage culture of Dunaliella salina for the production of β-carotene was feasible.

Key words: Dunaliella salina； &beta, -carotene； Two stage culture method；