食品科学 ›› 1998, Vol. 19 ›› Issue (4): 52-55.

• 食品科学 • 上一篇    下一篇

应用OPA柱前衍生法测定西红柿中的谷胱甘肽

 曹新志, 魏明   

  1. 四川省绵阳经济技术高等专科学校; 四川省绵阳市产品质量监督检验所;
  • 出版日期:1998-04-15 发布日期:2011-12-10

 CAO  Xin-Zhi, WEI  Ming   

  • Online:1998-04-15 Published:2011-12-10

摘要: 研究了采用OPA柱前衍生法测定西红柿中的谷胱甘肽。通过ODSC─18柱分离,流动相为60%的0.15mol/L乙酸钠溶液(pH7.0)和40%的甲醇。荧光检测器波长为:EX:340nm和EM:420nm。回收率为99.78-99.87%,变异系数(CV)为1.19%。

关键词: 邻苯二甲醛, 柱前衍生, 西红柿, 谷胱甘肽

Abstract:  A method for measurement of glutathione in tomato by OPA pre -- column deriration is described . The sample is treated simply through shivering, oscillating, centrifuging, deriration and separated by DOS C-- 18 columm. The mobile phase is 60% 0. 15mol/ L sodium acetdate (pH7. 0). The fluorescence detector wavelength is EX 340 nm and EM 420nm and its recorery is from 99. 78 to 99. 87%. Coefficient of variation is 1. 19% .

Key words: orthophthaladetyde Pre -- column deriation Tomato glutathione