食品科学 ›› 1998, Vol. 19 ›› Issue (9): 20-23.

• 食品科学 • 上一篇    下一篇

产菊糖酶固定化细胞包埋剂的筛选

曾胤新   

  1. 中国极地研究所
  • 出版日期:1998-09-15 发布日期:2011-12-12

Selection of carrier for Immobilized Cells with inulinase

 ZENG  Yin-Xin   

  • Online:1998-09-15 Published:2011-12-12

摘要: 把产菊糖酶的克鲁维酵母γ-85细胞固定于脱乙酰几了质、聚乙烯醇-海藻酸钠、琼脂、海藻酸钠、明胶等不同的载体上进行连续发酵,经过对凝胶的机械强度、固定化细胞的最适作用条件及其稳定性、酶活保留率和制作工艺等的综合比较,最后认为明胶是较好的载体。

关键词: 菊糖酶, 固定化细胞

Abstract: It is important for continuos fermentation to select a suitable camers of immobilized cells. We embedded Khuyveromyces sp. Y-85 cells with inulinase in various carriers such as chitosan, polyvinyl alcoholsodium alginate, agar, sodium alginate, gelatin, and utilized them for high fructose syrup production. After multiply compared with each other in the mechanical strengths of the prepared gels, the optimal reaction conditions and stabilities of immobilized cells, the retentions of inulinase activity and manufacturing procedures, the gelatin is selected as better carriers for immobilized yeast cells.

Key words: Inulinase Immobilized cells