食品科学 ›› 1997, Vol. 18 ›› Issue (4): 3-5.

• 食品科学 •    下一篇

葡萄糖酶传感器的探索研究

 左小明, 吴雪昌, 曾云中, 徐信业   

  1. 杭州大学生命科学学院; 杭州大学物理系
  • 出版日期:1997-04-15 发布日期:2011-12-20

Investigation of Glucose Biosensor Constructed with GOD

 ZUO  Xiao-Ming, WU  Xue-Chang, ZENG  Yun-Zhong, XU  Xin-Ye   

  • Online:1997-04-15 Published:2011-12-20

摘要: 将葡萄糖氧化酶(GOD)与辣根过氧化物酶(POD)用戊二醛共固定在半透膜上,以此作为生杨敏感元件制成传感器,在pH6.0,温度30℃,外加搅拌的条件下,对传感器的响应时间,线性测定范围及使用寿命等指标进行了测定,得到了良好的效果。酶膜的平均响应时间为2.5min,线性测定范围达0~300μg/ml,寿命约达15天。

关键词:  , GDD, POD, 酶膜, 生物传感器

Abstract: We used glucose oxidase (GOD) and horseradish peroxidase (POD) as material to construct glucose biosensor. On the conditions of pH 6. O, 3O℃, and stirring, the sensor worked effectively. Its average responsing time was 2~5 min, the linear measurable range was O~300 μg/ml, and the duration time was about 15 days.

Key words: GDD POD Enzyme membrane Biosensor