食品科学 ›› 2002, Vol. 23 ›› Issue (6): 132-135.

• 分析检测 • 上一篇    下一篇

爬山虎果中糖类物质含量测定方法的研究

 董爱文, 向中, 林桂艳, 袁子坤, 施立毛, 曾秋娟   

  1. 湖南武陵大学生物资源系
  • 出版日期:2002-06-15 发布日期:2012-02-13

Assay Study on Carbohy Drate Compound Contention Parthenoissus Tricosp idate Plach

 DONG  Ai-Wen, XIANG  Zhong, LIN  Gui-Yan, YUAN  Zi-Kun, SHI  Li-Mao, ZENG  Qiu-Juan   

  • Online:2002-06-15 Published:2012-02-13

摘要: 按邻甲苯胺比色法犤1犦盐酸水解法犤2犦的改进法测定爬山虎成熟果中糖类化合物的含量,按波长630nm犤1犦测定,检测线性范围0.02~0.50mg/ml,相关系数为0.9991,RSD=2.93%,回收率99.11%。此方法较其它方法操作简便准确、显色灵敏,重现性好,不受还原性物质的干扰,不必除去蛋白质。既能测定总糖,还能测定可溶性糖,因此很适合野生植物中糖类化合物含量的测定。

关键词: 爬山虎果, 改进法, 糖类化合物, 含量测定

Abstract: The carbohydrate compound content i n the ripe fruits of Parthenocissus Tricuspidate Plach was measured accordingtotheimprovedcolorimetricmethodofO -ToluidineandHydrolysis methodofHydrochloricacid.Thelinear measuringranges werefrom0.02~0.50mg /ml.Theinterratingcoefficien twas0.9991,RSD2.93%andtherecore ry rate99.11%.The methodhas characte ristics of easly operationto,acute color showing,wellreappearing and resistant to disturbance of reduction substan ces,Both the whole carbohydrate and soluble carbohydrate content were m easured,So it was also adaptable to the measur ing of carbohydrate chemical compou ndcontent inwild plants.

Key words: Parthenocissus Tricuspidate Plach , Improved method , Carbohydrate chemi cal compound , Con-tent measuring