食品科学 ›› 2002, Vol. 23 ›› Issue (3): 54-57.

• 基础研究 • 上一篇    下一篇

溶氧等参数对耐高温α-淀粉酶发酵的影响

 林剑, 郑舒文, 郭尽力, 鞠宝, 金海珠   

  1. 烟台大学化学生物理工学院
  • 出版日期:2002-03-15 发布日期:2012-02-13

Effect of Oxygen and pH on the Fermentation of the Thermostable α-Amylase Produced from Bacillus Lichenformis

 LIN  Jian, ZHENG  Shu-Wen, GUO  Jin-Li, JU  Bao, JIN  Hai-Zhu   

  • Online:2002-03-15 Published:2012-02-13

摘要: 利用耐高温α-淀粉酶的生产菌种—地衣芽孢杆菌IIY-1菌株,对其发酵过程中的各种参数的变化规律与控制方式进行了研究。实验结果表明,采用连续式的pH值的控制方式有利于提高耐高温α-淀粉酶的发酵水平,最后的发酵液的酶活力明显地高于脉动式的pH值控制方式。菌体在产酶阶段对发酵液的溶氧水平要求不高,但是溶氧浓度(DO值)不应低于10%。

关键词: 耐高温&alpha, -淀粉酶, 地衣芽孢杆菌, 溶氧浓度, 连续式和脉动式控制方式

Abstract: By using the HY-1 strain Bacillus Licheniformis to produce the thermostable α-amylase,the changes of every kind of parameters such as:the concentration of dissolved oxygen and pH of fermentation liquid were studied.The results of experiments showed that the use of continuous controlling method broth of pH valve had advantage over the pulsed controlling method of pH valve for the fermentation of thermostable α-amylase.The resulted broth of the former showed higher enzyme activity than the latfer.In the period of producing thermostable α-amylase,the cells could produce the thermostable α-amylase under rether low concentration of dissolved oxygen,butthislowleveloftheconcentrationofdissolvedoxygenshouldbeabove10%(DOvalve).

Key words: Thermostable α-amylase , Bacilluslicheniformis, Concentration ofdissolve doxygen, Continuous controlling, Method