食品科学 ›› 2002, Vol. 23 ›› Issue (3): 27-30.

• 基础研究 •    下一篇

构建高效降解胆固醇的融合乳酸菌的研究——融合子的鉴定及其在食品中应用

 王成涛, 牛天贵   

  1. 黑龙江八一农垦大学食品学院,中国农业大学食品学院
  • 出版日期:2002-03-15 发布日期:2012-02-13

Studies on Rebuilding Cholesterol-Degrading Lactic Acid Bacteria Using the Protoplast Fusion Technique--The Fusion Hybrids Defined and the Ability of Cholesterol-Degrading Observed

 WANG  Cheng-Tao, NIU  Tian-Gui   

  • Online:2002-03-15 Published:2012-02-13

摘要: 利用原生质体融合技术构建了两株融合子,通过比较C-T24-8、S-T13-37和三株亲本的胆固醇降解能力、凝乳能力、菌落特征、形状大小、抗生素抗性、生理生化特征和G+C%含量等遗传特征,证明它们确系区别于双亲的融合子。CT24-8和S-T13-37对胆固醇的降解率约为54%~78%,可降低食品基质中胆固醇约23%~45%,比亲本ST与C3有明显提高。并确定了气相色谱仪(GC)经程序升温测定乳酸的条件。

关键词: 融合子, 鉴定, 食品应用

Abstract: Fusion hybrids C-T24-8 and S-T13-37 were rebuilf by using the protoplast fusion technique.Affter Comparing the ability of cholesterol-translating,the ability of skim milk solidifying,the size,the physiologic characters,the biochemical characters and the G+C%of DNA and so on,it defined eventually that C-T24-8,and S-T13-37 were fusion hybrids of C3 and T12-1,ST and T12-1,Their rates of cholesterol-degrading were 54-78%,23-45%in medium of PY-CHO and food respectively.

Key words: Protoplast fusion , Define , Utilization