食品科学 ›› 2002, Vol. 23 ›› Issue (2): 78-80.

• 工艺技术 • 上一篇    下一篇

无蒸煮生淀粉糖化酶霉菌的选育

 丁立孝, 顾军, 倪新江, 刘聚国   

  1. 山东省莱阳农学院食品科学系,烟台大学生化系,山东莒县技术监督局
  • 出版日期:2002-02-15 发布日期:2012-02-13

Selective Breeding of Moulds Produced fromRaw -Starch -Saccarifying Gl ucoamylase without Steaming

 DING  Li-Xiao, GU  Jun, NI  Xin-Jiang, LIU  Ju-Guo   

  • Online:2002-02-15 Published:2012-02-13

摘要: 从23份土样中筛选出105株野生型霉菌,其中有10株具有较高的糖化生淀粉的能力,尤以A-4(Ⅱ)、C和E63株菌酶活力最高,分别达112562,825.1和825.1个酶活性单位;对C株菌进行紫外光诱变,从诱变后的菌株中筛选出有较大正向突变的两株菌株C(1)和C(5),与诱变前相比,C(1)菌的生淀粉糖化酶活力提高了27.3%,经生淀粉酒精发酵试验,相同条件下酒精度提高了22.4%;C(5)菌的生淀粉糖化酶活力提高了12.1%,酒精度提高了5.97%。

关键词: 无蒸煮, 生淀粉糖化酶, 霉菌, 选育

Abstract: wild strains of moulds were selected from23soil samples.Among themthere were10strains exhibiting higher sallharification effecfon s tarch.It was pointed that the enzyme activity of A -4(2),C and E6 had reached the highest activity,i.e,1125.2,825.1and825.1respectively,By inducing mutation c -strain withultraviolet ray ,two positive great mutationstrains c (1) andC(5) were obtained.Incomparisonwith th ose before inductionof mutation,th e activity of raw -starchsaccharifying enzyme (R?S?S?E)in C (1) was increasedby27.3%.In the same condition,the alcoholicdegree hadincreasedto22.4%.Bythealcoholicfermentationtest ofrawstarch,as inC (5) ,theactivityofR?S?S?Ewas increased by12.1%andalcoholic degree by5.97%.

Key words: Non -steaming , Raw -starch -digestio nglucomy lase , Moulds , Selective breeding