食品科学 ›› 2001, Vol. 22 ›› Issue (8): 46-49.

• 工艺技术 • 上一篇    下一篇

液态发酵产栀子蓝色素工艺条件研究

 李世杰,  方尚玲,  付向阳   

  1. 湖北工学院生物工程系
  • 出版日期:2001-08-15 发布日期:2012-02-13

Study on Gardenia Blue Liquid Fermentation Process Technology

 LI  Shi-Jie,   Fang-Shang-Ling,   Fu-Xiang-Yang   

  • Online:2001-08-15 Published:2012-02-13

摘要: 本实验室分离筛选到一株丝状真菌,可以利用栀子黄生产过程中吸附柱上洗脱液为原料,以液态发酵的方式转化为栀子蓝色素。通过正交试验优选到栀子蓝高产配方和培养条件为:蛋白胨4%,大豆浸出物1%,洗脱液7%;培养温度31℃,pH8.0,接种量5%(V/V),摇瓶装量120ml/500ml,摇床转速200r/min,培养周期96h。每升发酵液可获得色价高于50(E1%595)的栀子蓝色素12g以上。

关键词: 栀子蓝, 液态发酵, 正交试验

Abstract: A Filamental Fungus has been seperated and screened in our laboratory.It could be used in liquid fermentation to transport elution material from Gardenia yellow to Gardenia blue.Orthogonal experiment showed that the optimum Gardenia blue culture medium and culture condition swere:peptone 4%, soybean extract 1%, elution material 7%, pH8.0; culture temperature 31℃, inoculum 5%(V/V),culture broth quantity 200ml/500ml flask, shake rotation 200 r/min and fermentation period 96 hr. The culture can produce 12g Gardenia blue pigment (E1%595≥50) per liter fermented broth.

Key words: Gardenia blue Liquid fermentation Orthogonal experiment