食品科学 ›› 2001, Vol. 22 ›› Issue (11): 46-47.

• 工艺技术 • 上一篇    下一篇

茶叶粗多糖的提取及纯化研究

 周鹏, 谢明勇, 傅博强,  王远兴   

  1. 南昌大学生命科学与食品工程学院
  • 出版日期:2001-11-15 发布日期:2012-02-14

Study on Extraction and Purification of Coarse Tea Polysaccharides

 ZHOU  Peng, XIE  Ming-Yong, FU  Bo-Qiang,   Wang-Yuan-Xing   

  • Online:2001-11-15 Published:2012-02-14

摘要: 利用Sevag法脱游离蛋白、乙醇沉淀、二次透析等手段得到了初步纯化的茶叶粗多糖,该粗多糖经SephadexG100凝胶柱层析纯化,0.1mol/LNaCl洗脱,获得一较大组分,命名为TGP,通过凝胶渗透法测定其平均分子量为6×104道尔顿。

关键词: 茶叶粗多糖, 凝胶柱层析, TGP, 分子量

Abstract: In this paper, coarse tea polysaccharides were preliminarily purified by means of Sevag deprotein, ethanol precipitation, two times dialysis etc. A large component, nominated TGP, was fractionated from the polysaccharides by Sephadex G100 gel column chromatography, eluted by 0.1mol/L NaCl. The average molecular weight was determined to be 6.0× 104 Dol by gel permeation chromatography.

Key words:  , Coarse tea polysaccharides Gel column chromatography TGP Molecular weight;