食品科学 ›› 2012, Vol. 33 ›› Issue (21): 226-230.

• 生物工程 • 上一篇    下一篇

金针菇液体发酵产漆酶培养条件的研究

刘 苹,李 刚,温少红*   

  1. 烟台大学生命科学学院
  • 收稿日期:2011-09-06 修回日期:2012-08-09 出版日期:2012-11-15 发布日期:2012-11-09
  • 通讯作者: 温少红* E-mail:wenshaohong1@163.com
  • 基金资助:

    山东省优秀中青年科学家科研奖励基金;山东省科技攻关计划

Optimization of Liquid Culture Conditions for Laccase Production by Flammulina velutipes LP03

  • Received:2011-09-06 Revised:2012-08-09 Online:2012-11-15 Published:2012-11-09

摘要: 在液体培养条件下,考察碳源、氮源、Cu2+以及培养条件等因素对金针菇LP03产漆酶的影响。采用ABTS法测定金针菇LP03产漆酶酶活力。结果表明:以3g/100mL玉米粉为碳源,1g/100mL蛋白胨为氮源,添加1mmol/L的Cu2+,初始pH6.0、200mL/500mL三角瓶的装液量、培养温度28℃、摇床转速160r/min条件下培养,金针菇LP03的漆酶产量最高,菌株产酶活力为(1813.52±5.59)U/L,是优化前(479.87U/L)的3.78倍,且金针菇LP03菌体生长状况良好。

关键词: 金针菇, 漆酶, 培养条件

Abstract: The effect of carbon resource, nitrogen source, Cu2+ and other factors on laccase secretion by and cell growth of Flammulina velutipes LP03 were investigated. The optimum conditions were 3 g/100 mL corn flour, 1 g/100 mL peptone,0.5 g/100 mL glucose,1 mmol/L copper sulfate, initial pH 6.0, 200 mL/500 mL liquid medium, 28 ℃ and 160 r/min. The maximal enzyme activity was (1813.52 ±5.59) U/L, which was 3.78 times of that before optimization.

Key words: Flammulina velutipes, laccase, culture condition

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