构建食品级表达纳豆激酶的乳酸乳球菌重组菌株

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 208-212.

构建食品级表达纳豆激酶的乳酸乳球菌重组菌株

冯浩，余凤云，单凤娟，马婷，闫达中*

武汉工业学院生物与制药工程学院

收稿日期:2011-09-13 修回日期:2012-09-27 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 冯浩 E-mail:sqfenghao@sina.com
基金资助:
湖北省教育厅中青年项目

Construction of a Strain of Recombinant Lactococcus lactis Expressing Food-Grade Nattokinase

Received:2011-09-13 Revised:2012-09-27 Online:2012-11-15 Published:2012-11-09

摘要/Abstract

摘要： 构建整合型表达载体pFY008，以HisH基因作为同源重组的靶位点，通过同源重组将含有纳豆激酶原基因的表达盒PnisA-aprN整合到乳酸菌的基因组上，实现纳豆激酶在乳酸乳球菌中食品级的表达。构建的食品级基因工程菌安全性高、稳定性好，并赋予了其溶栓的功能。

关键词: 纳豆激酶, 同源重组, 乳酸乳球菌

Abstract: An integrative expression vector designated as pFY008 was constructed with HisH gene as a homologous recombination target site. For food-grade expression of nattokinase gene (aprN) in Lactococcus lactis, the expression cassette PnisA-aprN gene was integrated into the genome of Lactococcus lactis by homologous recombination. A food-grade recombinant Lactococcus lactis was constructed to produce a thrombolytics protein. The recombinant Lactococcus lactis was found to have fibrinolytic activity. Meanwhile, it had fine stability and high safety.

Key words: nattokinase, homologous recombination, Lactococcus lactis

中图分类号:

Q786

冯?浩，余凤云，单凤娟，马?婷，闫达中*. 构建食品级表达纳豆激酶的乳酸乳球菌重组菌株[J]. 食品科学, 2012, 33(21): 208-212.

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