食品科学 ›› 2012, Vol. 33 ›› Issue (23): 244-248.

• 生物工程 • 上一篇    下一篇

N+离子注入选育类胡萝卜素高产菌株及其固态发酵条件优化

刘 绍1,2,李 琦1,3,刘卉琳1,贾 涛4,谢达平1,*   

  1. 1.湖南农业大学生物安全科学技术学院 2.湖南农业大学食品科技学院 3.湖南利尔康生物有限公司 4.江苏省生产力促进中心理化测试中心
  • 收稿日期:2011-09-14 修回日期:2012-11-02 出版日期:2012-12-15 发布日期:2012-12-12
  • 通讯作者: 谢达平 E-mail:xiedaping508@163.com

Mutation Breeding of High-Yield Carotenoid Producing Rhodotorula mucilaginosa by N+ Implantation and Optimization of Solid-state Fermentation Conditions for Carotenoid Production

  • Received:2011-09-14 Revised:2012-11-02 Online:2012-12-15 Published:2012-12-12

摘要: 为获得类胡萝卜素高产菌株,利用N+注入方法对黏性红圆酵母菌RM-1进行诱变处理,结果表明,当注入能量为10keV时,最适诱变注入剂量为2.0×1014ion/cm2,筛选所得突变株RM-21产类胡萝卜素的能力明显提高,较出发菌株增加了60.85%。传代结果表明,传代10次突变株RM-21遗传性状稳定,可作为工业生产菌种。以玉米粉和稻米为原料,对突变株RM-21固态发酵产类胡萝卜素进行条件优化研究,结果表明,当m玉米粉:m稻米=3:1,含水量75%时,固态发酵最适条件为:葡萄糖4.0g/L、NH4NO3  2.5g/L、初始 pH6.0、接种量10%。在此基础上,对固态发酵产类胡萝卜素进行变温培养研究,结果表明,在96h的发酵时段中,0~84h温度控制在33℃,85~96h温度控制在28℃,类胡萝卜素产量达到7.04μg/g(以干基计),比恒温发酵提高了20.96%。

关键词: N+注入, 类胡萝卜素, 黏性红圆酵母, 固态发酵, 变温培养

Abstract: Rhodotorula mucilaginosa RM-1 was mutagenized by means of N+ implantation to obtain high-yield carotenoid producing mutants. The optimum dose of 10 keV N+ implantation was 2.0 × 1014 ion/cm2 and the best mutant (RM-21) revealed a 60.85% increase in carotenoid production when compared with the original strain. This mutant remained stable after the 10th passage and thus could be used for industrial production. Further investigations were conducted to optimize solid-state fermentation conditions for the production of carotenoid from corn flour and rice by mutant RM-21. The optimum fermentation conditions were determined as follows: a 3:1 (m/m) mixture of corn flour and rice with 75% added water as fermentation substrate, 4.0 g/L of glucose concentration, 2.5 g/L of NH4NO3 concentration, initial pH 6.0 and 10% of inoculum size. The fermentation time was 96 h and consisted of two stages: the fermentation temperature was 33 ℃ in the first 84 h and 28 ℃ in the following 12 h. Under these conditions, the yield of carotenoid was 7.04 μg/g dry matter, which revealed a 20.96% increase compared with constant temperature fermentation.

Key words: N+ implantation, carotenoid, Rhodotorula mucilaginosa, solid-state fermentation, temperature-shift culture

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