食品科学 ›› 2012, Vol. 33 ›› Issue (23): 240-243.

• 生物工程 • 上一篇    下一篇

有机溶剂稳定性蛋白酶发酵动力学研究

房耀维1,余 勃2,刘 姝1,王淑军1,*,吕明生1,焦豫良1,曹 纯1   

  1. 1.淮海工学院海洋学院,江苏省海洋资源研究院 2.南昌大学中德联合研究院
  • 收稿日期:2011-09-28 修回日期:2012-11-05 出版日期:2012-12-15 发布日期:2012-12-12
  • 通讯作者: 王淑军 E-mail:shujunwang86@163.com
  • 基金资助:

    江苏省高校自然科学基础研究面上项目

Fermentation Kinetics of Organic Solvent-stable Protease Production

  • Received:2011-09-28 Revised:2012-11-05 Online:2012-12-15 Published:2012-12-12

摘要: 利用分批发酵研究海洋细菌Bacillus aquaemaris DS1分泌有机溶剂稳定性蛋白酶的代谢特性,结果表明蛋白酶合成和菌体生长呈部分生长关联型。据分批发酵实验结果采用Logistic方程、Luedeking-Piret方程建立了描述菌株生长、产酶以及底物消耗分批发酵动力学模型。动力学模型计算值与实验值拟合良好,相对误差小于10%。

关键词: 有机溶剂稳定性脂肪酶, Bacillus aquaemaris DS1, 动力学模型, 海洋细菌

Abstract: Metabolic characteristics and the synthesis of organic solvent-stable protease during batch fermentation of Bacillus aquaemaris DS1 were investigated. The production of organic solvent-stable protease was partially associated with the growth of Bacillus aquaemaris DS1. Based on the results of batch fermentation experiments, the Logistic equation and Luedeking-Piret equation were used to establish kinetic models for bacterial growth, organic solvent-stable protease production and substrate consumption. Good agreement was observed between the experimental and the predicted data of residual sugar concentration with a relative error of less than 10%.

Key words: organic solvent-stable protease, Bacillus aquaemaris DS1, fermentation kinetic model, marine bacteria

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