绞股蓝热处理产物中活性成分的纯化与鉴定及其对A549细胞的抑制活性

食品科学 ›› 2012, Vol. 33 ›› Issue (23): 23-26.

绞股蓝热处理产物中活性成分的纯化与鉴定及其对A549细胞的抑制活性

朴香兰，杨静，吴倩，曾鸣，刘慧敏

中央民族大学中国少数民族传统医学研究院

收稿日期:2011-10-07 修回日期:2012-09-25 出版日期:2012-12-15 发布日期:2012-12-12
通讯作者: 朴香兰 E-mail:xlpiao@163.com
基金资助:
国家自然科学基金项目;国家自然科学基金项目;国家自然科学基金项目

Purification, Identification and Anti-A549 Cell Activity of Gypenosides from Thermally Processed Gynostemma pentaphyllum Leaves

Received:2011-10-07 Revised:2012-09-25 Online:2012-12-15 Published:2012-12-12
Contact: PIAO Xiang-Lan E-mail:xlpiao@163.com

摘要/Abstract

摘要： 目的：提取、分离、鉴定绞股蓝热处理产物中抑制肺癌A549细胞的活性成分。方法：绞股蓝叶在温度125℃、压力0.24MPa条件下，加热处理3h，用80%乙醇加热回流提取3h，利用活性跟踪方法，通过大孔树脂HP-20及反相柱等分离手段，分离抑制肺癌A549细胞的活性成分，并用核磁共振波普(1H-NMR、13C-NMR)、电喷雾质谱(ESI-MS)等数据鉴定有效成分。结果：绞股蓝热处理产物中分离得到2个达玛烷类皂苷，分别为绞股蓝皂苷Gypenoside L和Gypenoside LI，而且它们对肺癌A549细胞具有质量浓度依赖性的抑制活性(P＜0.05)，其IC50值分别为(48.43 ± 1.33)μg/mL和(34.35 ± 0.88)μg/mL。结论：热处理绞股蓝产物可提高对肺癌A549细胞的抑制作用，其中有效成分为达玛烷类皂苷Gypenoside L和Gypenoside LI。

关键词: 热处理绞股蓝, 肺癌A549细胞, 达玛烷类皂苷, Gypenoside L, Gypenoside LI

Abstract: Objective: To extract, separate and identify anti-A549 cell constituents from thermally processed Gynostemma pentaphyllum leaves. Methods: Gynostemma pentaphyllum leaves were processed at 125 ℃ and 0.24 MPa for 3 h and subjected to heat reflux extraction with 80% ethanol for 3 h. Anti-A549 cell constituents in the extract were separated using HP-20 macroporous adsorption resin and a reversed-phase column by means of bioassay-guided separation and identified by 1H-NMR, 13C-NMR and ESI-MS. Results: Two dammarane-type saponins were obtained and identified as gypenoside L and gypenoside LI, respectively. Both gypenosides had a concentration-dependent inhibitory effect on A549 cells with IC50 values of (48.43 ± 1.33) μg/mL and (34.35 ± 0.88) μg/mL, respectively. Conclusion: Thermally processed Gynostemma pentaphyllum leaves have a notable inhibitory effect on A549 cells, which is associated with the presence of gypenoside L and gypenoside LI.

Key words: thermally processed Gynostemma pentaphyllum, lung cancer A549 cells, dammarane-type saponin, gypenoside L, gypenoside LI

中图分类号:

朴香兰，杨?静，吴?倩，曾?鸣，刘慧敏. 绞股蓝热处理产物中活性成分的纯化与鉴定及其对A549细胞的抑制活性[J]. 食品科学, 2012, 33(23): 23-26.

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