食品科学 ›› 2012, Vol. 33 ›› Issue (19): 236-239.

• 生物工程 • 上一篇    下一篇

一株蓝色素产生菌的鉴定及色素特性

张志东,楚 敏,唐琦勇,宋素琴,王 玮,朱 静,顾美英   

  1. 新疆农业科学院微生物应用研究所
  • 收稿日期:2011-09-25 修回日期:2012-08-29 出版日期:2012-10-15 发布日期:2012-09-17
  • 通讯作者: 张志东 E-mail:zhangzheedong@sohu.com
  • 基金资助:

    农业部农业公益性行业科研专项经费项目;新疆维吾尔自治区自然科学基金资助项目

Identification of a Blue Pigment Producing Strain and Characterization of Produced Pigment

  • Received:2011-09-25 Revised:2012-08-29 Online:2012-10-15 Published:2012-09-17
  • Contact: Zhang Zhi-Dong E-mail:zhangzheedong@sohu.com

摘要: 为了确定一株筛选自低温污水的产蓝色素细菌B9-8分类地位和其色素的特性,通过对菌株的形态观察和16S rRNA基因序列比对分析,确定菌株B9-8归属为Janthinbacterium属菌,可能为新种。色素特性研究表明:该蓝色素在可见光区579nm波长处有最大吸收峰,具有较好的水溶性和有机溶性,较好的热稳定性和酸碱稳定性,但光稳定性较差,在自然光条件下放置3d色素损失61.3%。低浓度金属离子对色素的影响不大,而高浓度的金属离子,尤其是Al3+对色素的影响较大,损失率达73.1%,推测此色素极可能为紫色杆菌素。

关键词: 蓝色素, 菌种鉴定, 特性, 稳定性

Abstract: Purposes: To identify the classification position of a blue pigment producing strain named as B9-8 isolated from low temperature sewage and to characterize pigment produced by it. Methods: Based on morphological observation and 16 S rRNA sequence alignment, the strain was identified as a possible new member of the genus Janthinbacterium. Results: The blue pigment from this strain showed a maximum absorption peak at 579 nm and had good solubility in water and organic solvents. In addition, it was stable to heat and pH but unstable to light. After 3 d of exposure to natural sunlight a loss of 61.3% was found. Low concentrations of metal ions had little effects on its stability, whereas some metal ions at high concentrations, especially Al3+, had a great impact on its stability, resulting in a loss as high as 73.1%. Conclusion: Therefore, we speculated that the blue pigment might be violacein.

Key words: blue pigment, strain identification, properties, stability

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