两种有机磷农药对韭菜生理指标影响

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 131-135.

两种有机磷农药对韭菜生理指标影响

商飞飞1,2，王强2，陈文学1，吴莉宇2，彭齐3，仇厚援1,*

1.海南大学食品学院 2.省部共建国家重点实验室培育基地浙江省植物有害生物防控重点实验室，农业部农药残留检测重点实验室，浙江省农业科学院农产品质量标准研究所 3.浙江省农业科学院环境资源与土壤肥料研究所

收稿日期:2011-09-14 修回日期:2012-08-21 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 仇厚援 E-mail:Qiuhouyuan@sohu.com

Impact of Two Organophosphorus Pesticides on Physiological Indexes of Chinese Leek

Received:2011-09-14 Revised:2012-08-21 Online:2012-11-15 Published:2012-11-09

摘要/Abstract

摘要： 以两种有机磷农药毒死蜱、乐果分别喷施韭菜，在不同作用时间测得韭菜中可溶性蛋白质含量和谷胱甘肽-S-转移酶(GST)、谷胱甘肽过氧化物酶(GSH-Px)、酸性磷酸酯酶(ACP)、碱性磷酸酯酶(AKP)酶活力，得出酶活力变化动态曲线。结果表明：毒死蜱处理2h韭菜的可溶性蛋白含量升高，而乐果处理降低了可溶性蛋白质含量，1d与7d后两处理组可溶性蛋白含量均高于空白对照组，毒死蜱、乐果两组分别于14、11d恢复至正常水平；毒死蜱处理组韭菜GST活力一直处于被抑制状态，其中处理2h后最明显，乐果处理韭菜3d后GST活力明显高于空白对照组，11d略高于空白对照组，其他时间段也均受不同程度抑制；两组处理1d后GSH-Px活力均受到极显著抑制，3d时毒死蜱处理基本恢复正常，乐果处理则高于空白对照组，5d后两组均不同程度低于空白对照组，且低活性状态持续较长时间；两种有机磷农药对韭菜中的ACP、AKP酶活力影响不明显，而且毒死蜱与乐果两处理之间的差别也不大。结果说明：毒死蜱在韭菜中的作用时间略长于乐果，乐果处理组韭菜中GST、GSH-Px酶活性高于毒死蜱处理组，两种有机磷农药对韭菜ACP、AKP酶活性影响不大。

关键词: 毒死蜱, 乐果, 韭菜, 可溶性蛋白, GST, GSH-Px, ACP, AKP

Abstract: In this study, Chinese leeks were sprayed by chlorpyrifos and dimethoate, respectively. The content of soluble protein and the activities of glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), acid phosphatase (ACP), and alkaline phosphatase (AKP) in leeks were determined at different time interval after spraying. The results showed that soluble protein content in samples collected 2 h after chlorpyrifos spraying was increased, whereas the opposite result was observed when dimethoat was used. Both pesticides could result in an increase in soluble protein content in samples collected 1 d and 7 d after spraying compared with blank control group, and the chlorpyrifos and dimethoate treatment groups were restored to normal after 14 d and 11 d, respectively. GST activity was suppressed in Chinese leeks treated by chlorpyrifos, especially on the second day after spraying. GST activity was significantly higher in samples collected on the third day after dimethoate spraying and slightly higher those collected on the eleventh day than blank control group, and also inhibited to different extents at other time points. Similarly, GSH-Px activity was very significantly inhibited by chlorpyrifos and dimethoate and was basically restored to normal on the third after spraying, although an increased level of GSH-Px activity was observed for dimethoate treatment group compared with blank control group; on the fifth day, both treatment groups were lower to different extents than blank control group and then kept for a long time. The two pesticides did not have an obvious effect on ACP or AKP activities and showed only minor differences. The above finding clearly demonstrated that the effect of chlorpyrifos on Chinese leek was slightly more persistent than dimethoate, whereas GST and GSH-Px activities were increased by dimethoate compared with chlorpyrifos, and both pesticides had only minor effects on ACP and AKP activities.

Key words: chlorpyrifos, dimethoate, Chinese leek, soluble protein, GST, GSH-Px, ACP, AKP

商飞飞1,2，王?强2，陈文学1，吴莉宇2，彭?齐3，仇厚援1,*. 两种有机磷农药对韭菜生理指标影响[J]. 食品科学, 2012, 33(21): 131-135.

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