MWNTs/AChE新型生物传感器的制备及其在农药快速检测中的应用

摘要/Abstract

摘要： 本文采用层层自组装技术制备（壳聚糖/多壁碳纳米管/乙酰胆碱酯酶）多层膜，用以构建新型有机磷农药残留检测电流型生物传感器。利用扫描电子显微镜获得多层酶膜的显微结构，利用电化学工作站对新型生物传感器的性能进行测量。结果表明，生物传感器的多层酶膜的最适制备层数为5层；以氧化乐果作为有机磷农药模型，在0.25-1.50 μM 和 1.75-10.00 μM的氧化乐果浓度区间内，响应电流与抑制率呈现良好的线性关系，最低检出限达到10 nM；新型生物传感器具有良好的重现性和稳定性。

关键词: 乙酰胆碱酯酶, 生物传感器, 碳纳米管, 氧化乐果

Abstract: A novel amperometric biosensor for organophosphate pesticides based on multilayer films containing chitosan, multi-wall carbon nanotubes (MWNTs) and acetylcholinesterase (AChE) was developed. Micrographs of MWNTs/AChE multilayer films were obtained by scanning electron microscope, and properties of the resulting biosensors were measured by electrochemical measurements. Among the resulting biosensors, the biosensor based on five layers of multilayer films was best. Based on the inhibition of organophosphate pesticides on the AChE activity, using dimethoate as a model compound, the inhibition of dimethoate was proportional to its concentration ranging from 0.25 to 1.50 and from 1.75 to 10.00 μM, with a detection limit of 10 nM estimated at a signal-to-noise ratio of 3. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors.

中图分类号:

姜雪迟德富关桦楠. MWNTs/AChE新型生物传感器的制备及其在农药快速检测中的应用[J]. 食品科学, 2013, 34(4): 6-10.

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