紫外线与N+注入复合诱变选育曲酸高产菌株

食品科学 ›› 2013, Vol. 34 ›› Issue (1): 234-238.

紫外线与N+注入复合诱变选育曲酸高产菌株

凌帅1，刘咏1,*，姚建铭2，李俊2

1.合肥工业大学生物与食品工程学院 2.中国科学院合肥物质科学研究院

收稿日期:2011-10-08 修回日期:2012-12-17 出版日期:2013-01-15 发布日期:2013-01-07
通讯作者: 刘咏 E-mail:liuy99999@163.com

Mutation Breeding of High-Yield Kojic Acid-Producing Strains by UV Irradiation Combined with N+ Implantation

Received:2011-10-08 Revised:2012-12-17 Online:2013-01-15 Published:2013-01-07

摘要/Abstract

摘要： 为得到一株曲酸的高产菌株，从5种实验菌株中筛选得到1株米曲霉CICC-2336菌株，以其作为出发菌株，经过紫外诱变和氮离子注入诱变两种复合诱变，得到1株曲酸高产菌株，将其命名为Aspergillus oryzaeN11。该菌株经过传代6次后，性状稳定，产酸量基本不变。在接种量15%、30℃恒温、摇床180r/min条件下发酵培养7d后，其产酸量达到24.12g/L，比出发菌株提高101%。

关键词: 米曲霉, 曲酸, 紫外诱变, 氮离子注入诱变, 传代

Abstract: Aspergillus oryzae CICC-2336 was found to the best strain for the production of kojic acid followed by Huniang 3.811, AS 3.866, Yu3.811 and CICC-2337. A high-yield kojic acid-producing strain designated as N11 was obtained from the starting strain CICC-2336 by UV irradiation combined with N+ implantation. After the sixth passage, mutant N11 remained stable and its kojic acid production remained basically unchanged. The production of kojic acid was 24.12 g/L after 7 d of fermentation at 30 ℃ and a shaking speed of 180 r/min with an inoculum size of 15%, which was 101% higher than that of the starting strain.

Key words: Aspergillus oryzae, kojic acid, UV-induced mutation, N+ implantation-induced mutation, subculture

中图分类号:

TS201.3

凌?帅1，刘?咏1,*，姚建铭2，李?俊2. 紫外线与N+注入复合诱变选育曲酸高产菌株[J]. 食品科学, 2013, 34(1): 234-238.

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