紫甘薯汁抗肿瘤活性及诱导人肝癌细胞HepG2凋亡机制研究

食品科学 ›› 2013, Vol. 34 ›› Issue (1): 263-267.

紫甘薯汁抗肿瘤活性及诱导人肝癌细胞HepG2凋亡机制研究

陈永强，刘金娟，曹成亮，卞光凯，蒋继宏*

徐州师范大学江苏省药用植物生物技术重点实验室

收稿日期:2011-10-31 修回日期:2012-12-13 出版日期:2013-01-15 发布日期:2013-01-07
通讯作者: 蒋继宏 E-mail:jhjiang@xznu.edu.cn
基金资助:
杨木变色生防菌代谢物质抗变色真菌活性研究;江苏省沿海滩涂部分药用植物内生放线菌多样性与活性初步研究;新型放线菌素D 衍生物抗肿瘤效应及其分子机制研究

Anti-Tumor Activity of Purple Sweet Potato Juice and Its Apoptosis-Inducing Mechanism in HepG2 Cells

Received:2011-10-31 Revised:2012-12-13 Online:2013-01-15 Published:2013-01-07

摘要/Abstract

摘要： 目的：研究紫甘薯汁的抗肿瘤活性及其诱导肝癌细胞HepG2凋亡机制。方法：采用Alamar Blue、倒置显微镜、荧光显微镜、扫描电镜、琼脂糖凝胶电泳、RT-PCR和Western blotting法检测不同体积分数的紫甘薯汁对SGC-7901、HO-8910和HepG2细胞的增殖抑制作用及诱导人肝癌细胞HepG2凋亡机制研究。结果：与对照组相比，体积分数5%和10%的紫甘薯汁对SGC-7901、HO-8910和HepG2细胞具有抑制作用，呈体积分数依赖性；作用HepG2细胞48h后，能观察到细胞皱缩和凋亡小体以及凋亡细胞典型的梯状DNA条带。RT-PCR和Western blotting法检测结果显示，紫甘薯汁可以诱导HepG2细胞中Fas、FADD、Caspase-3、Caspase-8和p53 mRNA表达水平上调和蛋白表达量增加，Caspase-3、Caspase-8、Caspase-9的活性裂解片段明显增高且Caspase-3酶活性显著提高。结论：紫甘薯汁体外对肿瘤细胞的增殖具有一定抑制作用且通过细胞表面死亡受体途径诱导HepG2细胞凋亡，同时p53在此凋亡过程中也起着重要的作用。

关键词: 紫甘薯, 人肝癌细胞HepG2, 抗肿瘤活性, 细胞凋亡

Abstract: This study was undertaken to explore the anti-tumor activity of purple sweet potato juice and its apoptosis-inducing mechanism in HepG2 cells. The inhibitory effect of purple sweet potato juice at various concentrations on cell proliferation was examined by Alamar Blue assay. Cell apoptosis was determined by Hoechst 33258 fluorescence staining, scanning electron microscopy (SEM) and agarose gel electrophoresis. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting analysis were used to detect the expression of apoptosis-related factors in HepG2 cells treated with purple sweet potato juice at different concentrations for 24 h and 48 h, respectively. The results showed that the cell proliferation was inhibited by purple sweet potato juice in a dose-dependent manner. Cell shrinkage and apoptotic bodies after treatment for 48 h in HepG2 cells were observed. Typical DNA ladders in apoptotic cells were also observed. In addition, purple sweet potato juice up-regulated Fas, FADD, Caspase-3, -8 and p53 mRNA and protein expression levels. Moreover, active fragments of Caspase-3, -8 and -9 showed a significant increase, especially Caspase-3. Therefore, purple sweet potato juice can inhibit the proliferation of cancer cells and induce the apoptosis of HepG2 cells through extrinsic apoptotic pathway and p53 may also play an important role in this pathway.

Key words: purple sweet potato, HepG2, anti-tumor activity, apoptosis

中图分类号:

陈永强，刘金娟，曹成亮，卞光凯，蒋继宏*. 紫甘薯汁抗肿瘤活性及诱导人肝癌细胞HepG2凋亡机制研究[J]. 食品科学, 2013, 34(1): 263-267.

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