辅酶Q9对人类血管内皮细胞氧化损伤的影响

摘要/Abstract

摘要： 目的：研究辅酶Q9对人类血管内皮细胞氧化损伤的影响。方法：采用MTT法构建H2O2诱导的人脐静脉内皮细胞HUVEC氧化损伤模型，研究辅酶Q9对HUVEC细胞氧化损伤的预防、修复作用及其作用机理。结果：质量浓度为100μg/mL的辅酶Q9对由H2O2诱导的HUVEC细胞的氧化损伤有预防和修复功能。抑制HUVEC细胞中 ROS、MDA的增加是作用机制之一；同时，辅酶Q9预处理可有效减少H2O2对SOD酶的损伤，但对已损伤的SOD酶无修复作用。结论：辅酶Q9对H2O2造成的血管内皮细胞氧化损伤具有保护和修复功能。

关键词: 辅酶Q9, 血管内皮细胞, 氧化损伤, 保护, 修复

Abstract: Objective: To explore the protective effect of coenzyme Q9 against H2O2-induced oxidative damage in human vascular endothelial cells. Methods: An HUVEC (human umbilical vein endothelial cells) model of H2O2-induced oxidative damage was created by MTT assay and used to study the preventive effect and its mechanism of coenzyme Q9 against H2O2-induced oxidative damage. Results: Coenzyme Q9 at 100 μg/mL could exert preventive and repairing effects against H2O2-induced oxidative damage in HUVEC cells. The mechanism was partially due to that fact that the generation of ROS and MDA was inhibited by coenzyme Q9. Meanwhile, preventive treatment with coenzyme Q9 could effectively protect SOD against H2O2-induced damage but had no repairing effect. Conclusion: Coenzyme Q9 has protective and repairing effects against oxidative damage in human vascular endothelial cells.

Key words: coenzyme Q9, vascular endothelial cell, oxidative damage, protective effect, repairing effect

中图分类号:

于海宁1，楼肃文1，马?颀2，林秋生2，沈生荣2,*. 辅酶Q9对人类血管内皮细胞氧化损伤的影响[J]. 食品科学, 2012, 33(21): 314-317.

参考文献

[1] CHENG W, SONG C, ANJUM K M, et al. Coenzyme Q plays opposing roles on bacteria/fungi and viruses in Drosophila innate immunity [J]. International Journal of Immunogenetics, 2011, 38(4): 331-337.
[2] ROSA B M, CORDERO M D, ANTONIO S J, et al. Amitriptyline induces Coenzyme Q deficiency and oxidative damage in mouse lung and liver [J]. Toxicology Letters, 2011, 204(1): 32-37.
[3] OCHOA J J, PAMPLONA R, CARMEN R M, et al. Age-related changes in brain mitochondrial DNA deletion and oxidative stress are differentially modulated by dietary fat type and coenzyme Q10[J]. Free Radical Biology and Medicine, 2011, 50(9): 1053-1064.
[4] SOHAL R S, FORSTER M J. Coenzyme Q, oxidative stress and aging [J]. Mitochondrion, 2007, 7(S): S103-S111.
[5] ACWORTH I N, ULLUCCI, P A, GAMACHE, P H, ET AL. Determination of oxidized and reduced coQ10and coQ9 in human plasma/serum using HPLC-ECD [J]. Advanced Protocols in Oxidative Stress I, 2008, 477: 245-258.
[6] TANG P H. An improved LCEC method for simultaneous determination of coenzyme Q9 and coenzyme Q10 in HUMAN skeletal muscle [J]. Clinical Chemistry, 2008, 54(6): A143-A143.
[7] OCHOA J J, QUILES J, LOPEZ-FRIAS M, et al. Effect of lifelong coenzyme Q10 supplementation on aged-related oxidative stress and mitochondrial function in liver and skeletal muscle of rats fed on a polyunsaturated fatty acid (PUFA)-rich diet [J]. Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 2007, 62(11): 1211-1218.
[8] RODRIGUEZ-ACUNA R, BRENNE E, LACOSTE F. Determination of coenzyme Q10 and Q9 in vegetable oils [J]. Journal of Agricultural and Food Chemistry, 2008, 56(15): 6241–6245.
[9] MATTILA P; KUMPULAINEN J. Coenzymes Q9 and Q10: Content in foods and dietary intake [J]. Journal of Food Composition and Analysis, 2001, 14(4): 409-417.
[10] KUSUMOTO C, KINUGAWA T, MORIKAWA H, et al. Protection by exogenously added coenzyme Q(9) against free radical-induced injuries in human liver cells [J]. Journal of Clinical Biochemistry and Nutrition, 2010, 46(3): 244-251.
[11] 杨丽娟, 游育红, 林志彬等. 灵芝多糖肽对人脐静脉内皮细胞氧化损伤的保护作用[J]. 中国药理学通报, 2010, 26(5): 657-660.
[12] 龙石银, 黄良珠, 乔新惠等. 二苯乙烯苷对过氧化氢诱导内皮细胞凋亡的影响[J]. 中国动脉硬化杂志, 2011, 19(3): 181-186.
[13] 李伟东, 刘雪东, 蔡宝昌. 当归CO_2超临界萃取物对氧化应激损伤血管内皮细胞的保护作用. 南京中医药大学学报, 2010, 26(5): 365-367.
[14] VASTA V, SEDENSKY M, MORGAN P, et al. Altered redox status of coenzyme Q9 reflects mitochondrial electron transport chain deficiencies in Caenorhabditis elegans [J]. Mitochondrion, 2011, 11(1): 136-138.
[15] VALLS-BELLES V, TORRES C, MUNIZ PILAR, et al. Effect of beer consumption on levels of complex I and complex IV liver and heart mitochondrial enzymes and coenzymes Q9 and Q10 in adriamycin-treated rats [J]. European Journal of Nutrition, 2010, 49(3): 181-187.
[16] KUZELOVA M, ADAMEOVA A, SUMBALOVA Z, et al. The effect of simvastatin on coenzyme Q and antioxidant/oxidant balance in diabetic-hypercholesterolaemic rats [J]. General Physiology and Biophysics, 2008, 27(4): 291-298.
[17] NG M H, CHOO Y M, MA AN, et al. Determination of coenzyme Q9 and Q10 in Developing Palm Fruits [J]. Journal of the American Oil Chemists Society, 2009, 86(3): 201-205.
[18] LEKLI I, DAS S, DAS S, et al. Coenzyme Q9 provides cardioprotection after converting into coenzyme Q10 [J]. Journal of Agricultural and Food Chemistry, 2008, 56(13): 5331-5337.