食品科学 ›› 2013, Vol. 34 ›› Issue (3): 202-206.

• 生物工程 • 上一篇    下一篇

响应面法优化VC二步混合菌新菌系发酵培养基

高明1,吕淑霞2   

  1. 1. 沈阳农业大学
    2. 沈阳农业大学生物科学技术学院
  • 收稿日期:2011-10-21 修回日期:2013-01-04 出版日期:2013-02-15 发布日期:2017-12-29
  • 通讯作者: 吕淑霞 E-mail:lushuxia@hotmail.com
  • 基金资助:
    维生素C混合菌发酵中伴生菌的AP36活性蛋白基因克隆表达及其对产酸菌作用的分子机制研究;Vc二步发酵混合菌中伴生菌对产酸菌的作用机制研究

Optimization of Medium Components for Two-step Fermentation of Vitamin C Using Response Surface Methodology

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  • Received:2011-10-21 Revised:2013-01-04 Online:2013-02-15 Published:2017-12-29

摘要: 为提高VC二步发酵法中糖酸转化过程的产酸量,本实验室从新鲜牛奶中筛选出一株高效促产酸菌生长和产酸的伴生菌——枯草芽孢杆菌A9。在单因素试验基础上,采用响应面法对发酵培养基成分进行优化。结果表明,L-山梨糖、玉米浆、尿素的影响比较显著,最佳培养基条件为L-山梨糖90 g/L、尿素12.2 g/L、玉米浆14.2 g/L、碳酸钙4.1 g/L、硫酸镁0.2 g/L,可获得2-酮基-L-古龙酸产量为69.74 g/L,与理论值70.33 g/L的相对误差为-0.59 g/L。曲面回归方程与实验结果拟合性较好,此模型合理可靠,可用于实际预测。优化后的培养基比原培养基平均提高2-KGA产量17.4%

Abstract: In order to improve the 2-keto-L-gulonic acid production of the conversion process from L-sorbose to 2-keto-L-gulonic acid in vitamin C two-step fermentation. An efficient companion strain of Bacillus subtilis A9, which facilitating the growth of Gluconobacter oxydans and its acid production, was screened from fresh milk. Based on single factor experiments, the nutrient conditions of fermentation medium were optimized by response surface methodology. The results showed that the three factors of L-sorbose, corn steep liquor, urea affected 2-KGA production significantly. The optimal conditions were as follows(g/L): 90 L-sorbose, 14.2 corn steep liquor, 12.2 urea, 4.1 CaCO3, 0.2 MgSO4, and the yield of 2-keto-L-gulonic acid production was 69.74 g/L,with the theoretical 70.33 g/L for the relative error of -0.59 g/L. The established regression equation for 2-KGA production has a preferable goodness of fit. Therefore it is credible and can be used for practical prediction. The yield of 2-KGA increased by 17.4% under the optimized culture medium conditions comparing with the original medium.

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