食品科学 ›› 2013, Vol. 34 ›› Issue (3): 70-74.

• 基础研究 • 上一篇    下一篇

单癸月桂酸甘油酯的抑菌作用及机理研究

曾哲灵1,赵存洋2,罗春燕2   

  1. 1. 南昌大学食品科学与技术国家重点实验室219室
    2. 南昌大学食品科学与技术国家重点实验室217室
  • 收稿日期:2011-11-11 修回日期:2013-01-14 出版日期:2013-02-15 发布日期:2017-12-29
  • 通讯作者: 曾哲灵 E-mail:zlzenglx@163.com

Study on Antibacterial Function and Mechanism of Monolaurin and Monocaprin

  • Received:2011-11-11 Revised:2013-01-14 Online:2013-02-15 Published:2017-12-29

摘要: 对不同浓度的单癸月桂酸甘油酯的抑菌活性及其抑菌机理进行研究。采用滤纸片法对单癸月桂酸甘油酯进行细菌敏感性测试;二倍试管稀释法测定最低抑菌浓度(MIC);光电比浊法测定抑菌效果;二乙酸荧光素(FDA)染色实验研究其对细菌膜通透性的影响;分光光度法测定细菌胞内蛋白含量、细菌细胞壁脂多糖(LPS)含量、细菌菌悬液离心后上清液中核酸物质含量;原子吸收光谱法测定细菌菌悬液离心后上清液中胞内内容物K+、Ca2+、Mg2+含量;扫描电镜观测细菌表面形态变化。结果表明:单癸月桂酸甘油酯对革兰氏阳性菌及阴性菌均有较好的抑制效果,对金黄色葡萄球菌(G+)、枯草芽孢杆菌(G+)的抑制作用主要是破坏其细胞膜及细胞壁,对大肠杆菌(G-)的抑制作用主要是引起细胞壁外膜LPS外泄。

关键词: 单癸月桂酸甘油酯, 抑菌作用, 抑菌机理

Abstract: The aim of this work was to explore the antimicrobial activity and mechanism of monoglycerides containing monolaurin and monocaprin. Paper Disc Method was used to evaluate bacteria susceptibility of monolaurin and monocaprin. Two-Fold Dilution Method was used to test minimum inhibitory concentration (MIC), and Photoelectricity Turbidimetry Method was used to test antibacterial efficacy. Orescein diacetate (FDA) was used as fluorescent indicator to reflect the membrance permeability of testing bacteria after treating with monolaurin and monocaprin. Spectropbotometry Method was used to test the leak of internal substance of testing bacteria, such as: protein, nucleic acid, lipopolysaccharide, K+, Ca2+ and Mg2+. The change of cell surface was characterized using scanning electron microscope. Result showed that gram-positive and gram-positive bacteria were susceptive to monolaurin and monocaprin. Activity of monolaurin and monocaprin against Bacillus subtilis (G+) and Staphylococus aureus (G+) resulted from the destruction of cell wall and membrance caused by monolaurin and monocaprin. On the contrary, its antibacterial activity to Escherichia coil (G-) resulted from the leaking of lipopolysaccharide in the cell wall.